INVESTIGADORES
VAZQUEZ Elba Susana
congresos y reuniones científicas
Título:
Aberrant localization of heme oxygenase-1 in localized prostate cancer
Autor/es:
SACCA PAULA; MEISS ROBERTO; CASAS GABRIEL; MAZZA OSVALDO; BATLLE ALCIRA; NAVONE NORA; VAZQUEZ ELBA
Lugar:
County Orange, USA
Reunión:
Congreso; 96th Annual Meeting American Association for Cancer Research; 2005
Institución organizadora:
American Association for Cancer research
Resumen:
Recent evidence has suggested that proliferative inflammatory atrophy is a precursor of prostatic intraepithelial neoplasia and prostate cancer (PCa). The frequent association of lesions of proliferative inflammatory atrophy with chronic inflammation suggests that chronic or recurrent prostate inflammation may initiate and promote PCa development. Heme oxygenase-1 (HO-1) is an enzyme that has cytoprotective effect from immune-mediated injury either through prevention of oxidative damage or via a local immunomodulatory influence on infiltrating inflammatory cells. We hypothesize that modulation of HO-1 expression may play a role in the process of prostate carcinogenesis and PCa progression. We thus studied the expression and localization of HO-1 in 28 cases of organ confined, primary PCa obtained after radical prostatectomy and in 11 specimens with benign prostatic hyperplasia (BPH). We correlated our findings with the pathological grade of the specimen under study, the clinical stage at the time of surgery and the progression of the disease. It was previously reported that expression of p27 in less than 40% of prostate cancer cells had a significantly increased risk of biochemical recurrence after radical prostatectomy. We then also used p27 expression as an additional variable in this study. We assessed expression of HO1 and p27 by immunohistochemical analysis of formalin fixed, paraffin embedded tissue samples. We considered that HO-1 expression was positive when positive immunostaining was observed in the cytoplasm of 25% or more of the cells. Because HO1 is a cytoplasmic protein, nuclear staining was considered positive if any nuclei stained for HO-1. We found that cytoplasmic HO-1 expression was similar in prostate cancer cells (16/28, 57%) and non-neoplastic surrounding tissue (18/27, 67%), while was low in BPH (4/11, 36%, p