INVESTIGADORES
VAZQUEZ Elba Susana
congresos y reuniones científicas
Título:
Heme oxygenase -1: an emerging target in prostate cancer
Autor/es:
GUERON GERALDINE; FERRANDO MERCEDES; DE LUCA PAOLA; DE SIERVI ADRIANA; VAZQUEZ ELBA
Lugar:
Cracovia, Polonia
Reunión:
Congreso; HEME OXYGENASES 2007. THE 5TH INTERNACIONAL CONGRESS; 2007
Institución organizadora:
HEME OXYGENASES
Resumen:
Prostate cancer (PCa), one of the most common cancers in men, is considered a lethal malignancy with increasing incidence worldwide. Although PCa progression is well characterized by the androgen resistant phenotype, little is known about the genetic events of the malignant transformation. Heme Oxygenase 1 (HO-1) could counteract immune-mediated injury either through prevention of oxidative damage or via a local immunomodulatory influence on infiltrating inflammatory cells. Due to the key role that inflammation plays in the development of the tumor and subsequent metastasis, we hypothesize that modulation of HO-1 expression plays a critical role in PCa progression. To better understand the role of HO-1 in PCa, we assessed the expression of this enzyme in PCa cell lines. Western Blot analysis revealed low basal levels in the androgen independent cell line PC3 compared to the androgen sensitive cell line MDA PCa 2b. Treatment of PCa cell cultures with hemin (80 uM, 24h), a potent inducer of HO-1, increased HO-1 protein expression significantly for both cell lines. HO-1 transcriptional induction was also observed for both cell lines under hemin treatment by RT-PCR and qPCR. Moreover, hemin induced HO-1 promoter activity. We further analyzed HO-1 modulation effect on cell proliferation (3H-incorporation), apoptosis (AnnexinV-FITC), and cell cycle regulation. Treatment of PCa cell cultures with hemin had no significant effect on apoptosis but interestingly resulted in a 23% and 45% (p