INVESTIGADORES
VAZQUEZ Elba Susana
congresos y reuniones científicas
Título:
Heme oxygenase 1 (HO-1): an emerging target in prostate cancer
Autor/es:
GUERON GERALDINE; DE SIERVI ADRIANA; VAZQUEZ ELBA
Lugar:
Los Angeles, USA
Reunión:
Congreso; ANNUAL MEETING AMERICAN ASSOCIATION FOR CANCER RESEARCH AACR CENTENNIAL; 2007
Institución organizadora:
American Association for Cancer Research
Resumen:
<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US; mso-fareast-language:EN-US;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Prostate cancer (PCa) is one of the most common cancers in men. It is considered a lethal malignancy with increasing incidence worldwide. Although PCa progression is well characterized by the androgen resistant phenotype, little is known about the genetic events of the malignant transformation. Androgen deprivation induces changes in the phenotype of PCa followed by alterations in protein expression. Recent evidence has suggested that proliferative inflammatory atrophy is a precursor of prostatic intraepithelial neoplasia and PCa. Heme Oxygenase 1 (HO-1) the inducible isoform of the rate-limiting enzyme in the degradation of heme, could counteract immune-mediated injury either through prevention of oxidative damage or via a local immunomodulatory influence on infiltrating inflammatory cells. Due to the key role that inflammation plays in the development of the tumor and subsequent metastasis, we hypothesize that modulation of HO-1 expression plays a critical role in PCa progression. To better understand the role of HO-1 in PCa, we assessed the expression of this enzyme in PCa cell lines. Western Blot analysis revealed low basal levels in the androgen independent cell line PC3 compared to androgen sensitive cell lines (LNCaP and MDA PCa 2b). We further analyzed HO-1 modulation effect on cell proliferation (3H-incorporation) and invasion (Matrigel invasion chambers). Treatment of PC3 cell cultures with hemin (70 µM, 24h), a potent inducer of HO-1, resulted in a 20% (p