INVESTIGADORES
VAZQUEZ Elba Susana
congresos y reuniones científicas
Título:
Expression of caveolin-1 in cavernous tissue on a denervated animal model. Impact of Sildenafil
Autor/es:
E. BECHER; J. TOBLLI; C. NOLAZCO; C. ROSENFELD; H. GROSMAN; E. VAZQUEZ; C. CATRUONOVO; O. MAZZA
Lugar:
Orlando, Florida USA
Reunión:
Congreso; AMERICAN UROLOGY ASSOCIATION ANNUAL MEETING; 2008
Institución organizadora:
AMERICAN UROLOGY ASSOCIATION
Resumen:
Introduction: Radical pelvic surgery is a major cause of erectile dysfunction; it is related to the extent of the neural injury and might be influenced by early pharmacological intervention. Caveolae are specialized plasma membrane invaginations on the surface of most cells involved in endocytosis. Animal studies showed that aging and hypertension decreases trabecular SM/collagen ratio and as well as the concentration of caveolae and hence, a reduced expression of caveolin-1 (Cav-1), a caveolar protein present in cavernosal tissue. Objective: to determine de expression of Cav-1 as marker of cavernous tissue damage and to evaluate the impact of early sildenafil administration in animal models of partial and total penile denervation. Methods: 36 adult Sprague-Dawley rats were divided into 6 groups of bilateral, unilateral penile denervation and sham surgery with and without daily administration of 10 mg/kg of sildenafil. The animals were killed 7 weeks after the procedure and corporal tissue was analyzed with western blotting and immuno-staining with antibodies against Cav-1 and alpha-actine (standard marker). Results: Mean values for Cav-1 and actin were: Bilat: C:1.4 A:3.9 (p