Effect of a mixture of GOS/FOS® on calcium (Ca) and phosphorus (P) absorption and bone retention in ovariectomized osteopenic rats fed a low calcium diet.

BRYK; ZENI

Roma

Congreso; 43rd. Annual European Calcified Tissue Society Congress.; 2016

European Calcified Tissue Society

Dietary Ca insufficiency is a common finding, independently of socio-economic status. A low Ca intake (CaI) increases bone turnover leading to bone loss. This effect becomes more important during estrogen withdrawing. We previously found that a mixture of galactooligosaccharides (GOS) and fructooligosaccharides (FOS) enhances Ca and P absorption (Abs), being a suitable tool to optimize its bioavailability and consequently bone health. The present study evaluated the effect of feeding a low Ca diet containing a mixture of GOS/FOS (9:1) on Ca and P Abs and bone retention in female Wistar osteopenic rats. Rats were ovariectomized (OVX) or SHAM operated and remained untreated during a 45 day-period. Then, they were randomly assigned to received one of the following diets prepared according to the American Institute of Nutrition formulation for maintenance (AIN´93): AIN´93 (C) or AIN´93+2.5% GOS/FOS® (P) containing 0.5% or 0.3% of Ca (groups: C0.5%, C0.3%, P0.5% and P0.3%, respectively) for an additional period of 45-days. Dietary consumption was evaluated 3 times per week. Body weight (BW) was recorded weekly. Ca and P Abs percentage (%) were determined at the beginning and during the last 3 days of the experience. At the end of the study (tf) CTX (ng/mL): bone alkaline fosfatase (BALP) (IU/L), total skeleton bone mineral content/BW (tsBMC) (g/100gBW) and density (tsBMD) (mg/cm2), tibia and spine BMDs and Growth plate cartilage height (GPC.Th) (µm), were evaluated. Results at tf (mean±SD): C0.5%; C0.3%; P0.5%; P0.3%, respectively (Different letters indicate a p