INVESTIGADORES
ZENI Susana Noemi
congresos y reuniones científicas
Título:
Calcium intake affects body composition, bone metabolism and insulin resistance in spontaneously obese rats
Autor/es:
MAROTTE C; BRYK G; OLGUIN M;; LUCERO DM; SCHREIER L; FRIEDMAN S; DE PORTELLA ML; ZENI S
Lugar:
SAN DIEGO
Reunión:
Congreso; Asbmr; 2011
Resumen:
CALCIUM INTAKE AFFECTS BODY COMPOSITION, BONE METABOLISM AND INSULINE RESISTENCE IN SPONTANEOUS OBESE RATS.
Marotte C1,5,6, Bryk G1,5, Olguin M3, Lucero DM4, Schreier L4, Friedman SM6, Portela ML2, Zeni SN1,5,6.
1Sección Osteopatías Médicas. Hospital de Clínicas. 2Cátedra de Nutrición y Bromatología. Facultad de Farmacia y Bioquímica, UBA. 3Universidad Nacional de Rosario. 5Laboratorio de lípidos y lipoproteínas. Depto. Bioquímica Clínica. Facultad de Farmacia y Bioquímica, UBA. 5 Cátedra de Bioquímica General y Bucal. Facultad de Odontología. UBA. 6CONICET.
MB Zemel theory postulated in 2004: ?A high CaI would be a preventive factor for Ob/OW development?. Objective: to evaluate the effect of feeding different Ca content diet on body composition, bone metabolism and insulin-resistance in rats that spontaneously develop Ob in adolescent state. Female IIMbb rats fed from pregnancy one of 3 isocaloric diets only varied in Ca content: 0.9g% (HG), 0,5g% (NG) and 0,2g% (LG). At weaning male offspring continued feeding the same mother diet till 50 days of age (Tf). Results (mean ±SD), groups HG, NG & LG, respectively are shown in the table:
HG
NG
LG
Method
Food intake
13.51±1.55
12.26±1.80
13.69±1.63
-
Ca Intake (CaI)
221.0±15.0a
126.0±44.0b
46.0±9.0c
-
% Ca Absorption
64±6a
87±1b
91±9c
[(ICa? Fecal)/ICa] x100.
Body Weight (BW) (g)
195±9a
227±13b
262±7c
-
%Water
69.2±4.2
70.0±2.8
66.7±3.3
AOAC
%Protein (g/100g BW)
14.3±1.7
14.2±1.9
15.9±2.1
AOAC
%Fat
14.2±0.9a
13.7±0.9a
15.9±0.6b
AOAC
BMC (g/100g BW)
1.09±0.02a
1.29±0.08b
0.83±0.03c
DXA
P (mg/100g BW)
532±22a
520±31a
882±15b
UV-Colorimetry
BGP (ng/dl)
279±73a
375±46b
478±45c
ELISA
sCTX (mg/dl)
64.9±22.5a
71.9±12.4ab
80.6±14.1b
ELISA
Glucose (mg/dl)
111.8±25.5a
151.9±21.9b
207.0±11.0c
Colorimetry
Insulin (ng/ml)
1.92±0.75a
4.07±0.93b
6.87±2.24c
ELISA
Different letters: p<0.05. LG increased BW by the increment in body fat. An inverse relationship between CaI and insulin resistance was observed. Conclusions: The low CaI not only affects bone metabolism but also body composition and insulin resistance in spontaneous obese rats. UBACyT091.