INVESTIGADORES
ZENI Susana Noemi
congresos y reuniones científicas
Título:
Calcium intake affects body composition, bone metabolism and insulin resistance in spontaneously obese rats
Autor/es:
MAROTTE C; BRYK G; OLGUIN M;; LUCERO DM; SCHREIER L; FRIEDMAN S; DE PORTELLA ML; ZENI S
Lugar:
SAN DIEGO
Reunión:
Congreso; Asbmr; 2011
Resumen:
CALCIUM INTAKE AFFECTS BODY COMPOSITION, BONE METABOLISM AND INSULINE RESISTENCE IN SPONTANEOUS OBESE RATS. Marotte C1,5,6, Bryk G1,5, Olguin M3,  Lucero DM4, Schreier L4, Friedman SM6, Portela ML2, Zeni SN1,5,6. 1Sección Osteopatías Médicas. Hospital de Clínicas. 2Cátedra de Nutrición y Bromatología. Facultad de Farmacia y Bioquímica, UBA. 3Universidad Nacional de Rosario. 5Laboratorio de lípidos y lipoproteínas. Depto. Bioquímica Clínica. Facultad de Farmacia y Bioquímica, UBA. 5 Cátedra de Bioquímica General y Bucal. Facultad de Odontología. UBA. 6CONICET. MB Zemel theory postulated in 2004: ?A high CaI would be a preventive factor for Ob/OW development?. Objective: to evaluate the effect of feeding different Ca content diet on body composition, bone metabolism and insulin-resistance in rats that spontaneously develop Ob in adolescent state. Female IIMbb rats fed from pregnancy one of 3 isocaloric diets only varied in Ca content: 0.9g% (HG), 0,5g% (NG) and 0,2g% (LG). At weaning male offspring continued feeding the same mother diet till 50 days of age (Tf). Results (mean ±SD), groups HG, NG & LG, respectively are shown in the table:   HG NG LG Method Food intake 13.51±1.55 12.26±1.80 13.69±1.63 - Ca Intake (CaI) 221.0±15.0a 126.0±44.0b 46.0±9.0c - % Ca Absorption 64±6a 87±1b 91±9c [(ICa? Fecal)/ICa] x100.   Body Weight (BW) (g) 195±9a 227±13b 262±7c - %Water 69.2±4.2 70.0±2.8 66.7±3.3 AOAC %Protein (g/100g BW) 14.3±1.7 14.2±1.9 15.9±2.1 AOAC %Fat 14.2±0.9a 13.7±0.9a 15.9±0.6b AOAC BMC (g/100g BW) 1.09±0.02a 1.29±0.08b 0.83±0.03c DXA P (mg/100g BW) 532±22a 520±31a 882±15b UV-Colorimetry BGP (ng/dl) 279±73a 375±46b 478±45c ELISA sCTX (mg/dl) 64.9±22.5a 71.9±12.4ab 80.6±14.1b ELISA Glucose (mg/dl) 111.8±25.5a 151.9±21.9b 207.0±11.0c Colorimetry Insulin (ng/ml) 1.92±0.75a   4.07±0.93b 6.87±2.24c ELISA   Different letters: p<0.05. LG increased BW by the increment in body fat. An inverse relationship between CaI and insulin resistance was observed. Conclusions: The low CaI not only affects bone metabolism but also body composition and insulin resistance in spontaneous obese rats. UBACyT091.