Comparetive bone turnover in wistar (W) and obese (Ob) growing rats, fed different dietary calcium content.

MAROTTE C; WEISSTAUB A; SOMOZA J; DE PORTELA ML; ZENI SN,

Estocolmo

Congreso; 39th. European Calcified Tissue Simposium (ECTS); 2012

Abstract: Calcium (Ca) intake, bone turnover and obesity are interrelated. A low Ca intake increases bone turnover and it is related to obesity development. Moreover, obesity could affect bone metabolism. The objective was to compare the effect of feeding a low or normal Ca diet (LCa and NCa, respectively) on body weight and composition, bone turnover markers and insulin levels in W and genetically modified Ob rats during growth. Female W and Ob adult rats were mated and fed during pregnancy and lactation diets prepared according to AIN´93 recommendations. At weanling, male pups continued feeding the same diet ?ad libitum? till 50 days of age. Food consumption and body weight (BW) were recorded 3times/week. At the end of the experience total body ashes (AOAC methods), serum Ca and phosphorus (P), bone alkaline phosphatase (bAP), osteocalcin (BGP), C-telopeptide of type 1 collagen (CTX) and Insulin (ELISA) were determined. Results (mean± SD) were: W LCa (n=8) Ob LCa (n=10) W NCa (n=10) Ob NCa (n=11) sCa (mg/dl) 8.2±0.9a 9.7±0.5a 8.3±0.6a 9.5±0.3a sP (mg/dl) 9.2±0.5a 8.9±0.7a 8.2±1.0a 8.8±0.4a bAP (IU/L) 85±24a 82±17a 81±7a 88±17a BGP (ug/ml) 1046±167a 661±84b 799±171b 453±71c SCTX (mg/ml) 84.6±17.0a 69.7±3.3b 88.3±16a 98.5±27.6a Insulin (mg/dl) 1.34±0.9a 7.75±1.66b 0.14±0.02c 4.75±2.44d Body ashes (g/100 body weight) 2.13±0.33a 2.01±0.21b 3.21±0.33c 2.59±0.18d Different letters indicate p