INVESTIGADORES
ZENI Susana Noemi
congresos y reuniones científicas
Título:
Body composition, bone metabolism and insuline resistence interrelationship in spontaneous obese rats feeding different calcium content
Autor/es:
MAROTE C; WEISSTAUB A; GONZALES CHAVES M; BRYK G; OLGUIN M,; LUCERO DM; SCHREIER L; DE PORTELA ML; ZENI S
Lugar:
ATHENAS
Reunión:
Congreso; 38th. European Calcified Tissue Simposium (ECTS).; 2011
Resumen:
BODY COMPOSITION, BONE METABOLISM AND INSULINE RESISTENCE INTERRELATIONSHIP IN SPONTANEOUS OBESE RATS FEEDING DIFFERENT CALCIUM CONTENT. Marotte C1,5, Weisstaub A2, Gonzales Chaves MMS1,2,5, Bryk G1, Olguin M3, Lucero DM4, Schreier L4, Portela ML2, Zeni SN1,2,4. 1Sección Osteopatías Médicas. Hospital de Clínicas. 2Cátedra de Nutrición y Bromatología. Facultad de Farmacia y Bioquímica, UBA. 3Universidad Nacional de Rosario. 5Laboratorio de lípidos y lipoproteínas. Depto. Bioquímica Clínica. Facultad de Farmacia y Bioquímica, UBA. 5CONICET. Consistent with our previous findings, the Nutrition and Health National Survey (ENNyS) conducted in Argentina during 2004-2006 has demonstrated a high calcium intake (CaI) deficiency. Our later analysis of the Survey has shown an inverse relationship between CaI and obesity/overweight (Ob/OW) incidence, data similar to that of NHANES III (USA). These findings are in agreement with the MB Zemel theory that postulates (2004): “A high CaI would be a preventive factor for Ob/OW development”. On these bases we evaluated the effect of feeding different Ca content diet on body composition, bone metabolism and insulin-resistance in rats that spontaneously develop Ob in their adolescent state. Female adult IIMbb rats were fed from pregnancy one of the three experimental isocaloric diet that only varied in their Ca content: 0.9 g % (HG), 0,5 g% (NG) and 0,2 g% (LG) . At weaning male offspring continued feeding the same mother diet till 50 days of age (Tf). Food consumption and body weight (BW) were evaluated weekly and Ca absorption percentage (%CaAbs) in the three last days of experience. At the end of the experience (Tf) body composition (water, lipids, protein and ashes) (by AOAC methods), bone mineral density (BMD) and content (BMC) (by DXA), body Ca and Phosphorus (P) content and serum bone biochemical markers levels: bone alkaline phosphates (bALP), osteocalcin (BGP) and C-terminal telopeptide collagen type I (CTX) (by ELISA) and serum insulin and glucose levels were determined. Results (mean ±SD): groups HG, NG and LG respectively: BW (g); 195±9a; 227± 13b; 262±7c; % CaAbs; 64±6 a; 87±1 b; 91±9 c; ASHES (g/100g BW); 2.4±0.1a; 2.2±0.1a; 1.5±0.1b; Ca CONTENT (mg/100g BW); 620±34a; 640±22a; 394±40b P CONTENT (mg/100g BW); 532±22 a; 520±31 a; 882±15b; % BODY FAT; 14.2±0.9a; 13.7±0.9a; 15.9±0.6bç; BMC (g/g BW); 1.09±0.02 a; 1.29±0.08 b; 0.83±0.03 c; BGP (ng/dl); 279±73a; 375±46b; 478±45c; bALP (IU/L); 115±57 a; 143±46 a; 98±39 b; sCTX (ng/dl); 64.9±22.5a; 71.9±12.4 a,b; 80.6±14.1b; Glucose (mg/dl); 111. 8±25.5a; 151.9±21.9b;207.0±11.0c; Insulin (ng/ml); 1.92±0.75a; 4.07±0.93b; 6.87±2.24c. Different letters indicate a p