Protein and vitamin D deficiencies induce osteopenia in female rats

MARROTE C, ; GOMES CHAVES MMS, ; MANDALUNIS P, ; FRIEDMAN SM, ; ZENI SN

Viena

Congreso; 36th European Calcified Tissue Symposium; 2009

ECTS

Previously we found that a low dietary protein intake in OVX rats induces a severe osteopenia. In the present study we investigated the effect of anticatabolic treatment with the aminobisphosphonate, S410 Abstracts / Bone 44 (2009) S339–S450 olpadronate (OPD) in rats feeding a low protein, normal calcium diet. A total of 30 Wistar adult rats (300±50 g) were studied, 20 rats were ovariectomized (OVX) and 10 were SHAM operated. During 15 days post-surgery all rats were fed a commercial diet (Granave SA, Bs.As.) which supplied protein 15%, vitamin D 200UI% and 0.6% calcium. After that rats were divided in 4 groups and for an additional 60 days they received: G1: OVX+2.5% casein diet. G2: OVX+2.5% casein diet+8 ìg OPD/100 g rats once a week (intraperitoneally). G3: SHAM+commercial diet (protein 15%). At baseline (Tb) and at the end of the experience (Tf) total skeleton bone mineral content (BMC: mg) and density (BMD: mg/ cm2)) were measured by DXA (Small animal Software, Lunar DPX). Proximal tibia BMD (BMDpt) was evaluated using a specific ROI and changes in BMC and BMDpt between Tf and Tb was calculated (Ch. BMC and Ch.BMDpt, respectively). Serum calcium (Ca: mg/dl), phosphorus, bone alkaline phosphatase (bALP: IU/L) and osteocalcin (BGP: ng/ml) were also evaluated. At Tf bone volume of tibia (BV/TV: %) was measured by histology. Results (mean±standard error):ìg OPD/100 g rats once a week (intraperitoneally). G3: SHAM+commercial diet (protein 15%). At baseline (Tb) and at the end of the experience (Tf) total skeleton bone mineral content (BMC: mg) and density (BMD: mg/ cm2)) were measured by DXA (Small animal Software, Lunar DPX). Proximal tibia BMD (BMDpt) was evaluated using a specific ROI and changes in BMC and BMDpt between Tf and Tb was calculated (Ch. BMC and Ch.BMDpt, respectively). Serum calcium (Ca: mg/dl), phosphorus, bone alkaline phosphatase (bALP: IU/L) and osteocalcin (BGP: ng/ml) were also evaluated. At Tf bone volume of tibia (BV/TV: %) was measured by histology. Results (mean±standard error):2)) were measured by DXA (Small animal Software, Lunar DPX). Proximal tibia BMD (BMDpt) was evaluated using a specific ROI and changes in BMC and BMDpt between Tf and Tb was calculated (Ch. BMC and Ch.BMDpt, respectively). Serum calcium (Ca: mg/dl), phosphorus, bone alkaline phosphatase (bALP: IU/L) and osteocalcin (BGP: ng/ml) were also evaluated. At Tf bone volume of tibia (BV/TV: %) was measured by histology. Results (mean±standard error): Table 1 OVX+Vh OVX+OPD SHAM bALP 74±11a 50±5b 45±4b BGP 131.0±15.1a 111.2±12.7b 178±55c Calcium 9.6±0.1a 8.7±0.1b 10.3±0.1c Ch.BMC −253±19a 1512±343b 2706±556c Ch.BMDpt 0.0±0.4a 22.0±2.1b 59.2±5.2c BV/TV (%). 2.9±0.2a 7.1±0.9b−253±19a 1512±343b 2706±556c Ch.BMDpt 0.0±0.4a 22.0±2.1b 59.2±5.2c BV/TV (%). 2.9±0.2a 7.1±0.9bAbstracts / Bone 44 (2009) S339–S450 olpadronate (OPD) in rats feeding a low protein, normal calcium diet. A total of 30 Wistar adult rats (300±50 g) were studied, 20 rats were ovariectomized (OVX) and 10 were SHAM operated. During 15 days post-surgery all rats were fed a commercial diet (Granave SA, Bs.As.) which supplied protein 15%, vitamin D 200UI% and 0.6% calcium. After that rats were divided in 4 groups and for an additional 60 days they received: G1: OVX+2.5% casein diet. G2: OVX+2.5% casein diet+8 ìg OPD/100 g rats once a week (intraperitoneally). G3: SHAM+commercial diet (protein 15%). At baseline (Tb) and at the end of the experience (Tf) total skeleton bone mineral content (BMC: mg) and density (BMD: mg/ cm2)) were measured by DXA (Small animal Software, Lunar DPX). Proximal tibia BMD (BMDpt) was evaluated using a specific ROI and changes in BMC and BMDpt between Tf and Tb was calculated (Ch. BMC and Ch.BMDpt, respectively). Serum calcium (Ca: mg/dl), phosphorus, bone alkaline phosphatase (bALP: IU/L) and osteocalcin (BGP: ng/ml) were also evaluated. At Tf bone volume of tibia (BV/TV: %) was measured by histology. Results (mean±standard error):ìg OPD/100 g rats once a week (intraperitoneally). G3: SHAM+commercial diet (protein 15%). At baseline (Tb) and at the end of the experience (Tf) total skeleton bone mineral content (BMC: mg) and density (BMD: mg/ cm2)) were measured by DXA (Small animal Software, Lunar DPX). Proximal tibia BMD (BMDpt) was evaluated using a specific ROI and changes in BMC and BMDpt between Tf and Tb was calculated (Ch. BMC and Ch.BMDpt, respectively). Serum calcium (Ca: mg/dl), phosphorus, bone alkaline phosphatase (bALP: IU/L) and osteocalcin (BGP: ng/ml) were also evaluated. At Tf bone volume of tibia (BV/TV: %) was measured by histology. Results (mean±standard error):2)) were measured by DXA (Small animal Software, Lunar DPX). Proximal tibia BMD (BMDpt) was evaluated using a specific ROI and changes in BMC and BMDpt between Tf and Tb was calculated (Ch. BMC and Ch.BMDpt, respectively). Serum calcium (Ca: mg/dl), phosphorus, bone alkaline phosphatase (bALP: IU/L) and osteocalcin (BGP: ng/ml) were also evaluated. At Tf bone volume of tibia (BV/TV: %) was measured by histology. Results (mean±standard error): Table 1 OVX+Vh OVX+OPD SHAM bALP 74±11a 50±5b 45±4b BGP 131.0±15.1a 111.2±12.7b 178±55c Calcium 9.6±0.1a 8.7±0.1b 10.3±0.1c Ch.BMC −253±19a 1512±343b 2706±556c Ch.BMDpt 0.0±0.4a 22.0±2.1b 59.2±5.2c BV/TV (%). 2.9±0.2a 7.1±0.9b−253±19a 1512±343b 2706±556c Ch.BMDpt 0.0±0.4a 22.0±2.1b 59.2±5.2c BV/TV (%). 2.9±0.2a 7.1±0.9b