INVESTIGADORES
ZENI Susana Noemi
congresos y reuniones científicas
Título:
Body composition and bone density and its remodelling markers: a follow up study in a rat model for the dietary management of nutritional dwarfing
Autor/es:
SUAREZ CRISTINA; FERREIRA MONTEIRO ANDREA; RODRIGUEZ PATRICIA; ZENI SUSANA N; FRIEDMAN SILVIA
Lugar:
San Diego 2-6 abril de 2005
Reunión:
Congreso; Experimental Biology; 2005
Institución organizadora:
FASEB
Resumen:
The purpose of this work was to study in an experimental rat model the effect of the long-term -from weaning throughout reproductive cycle-, intake of low calcium and/or low fat diet on body composition (BC)  and tibiae mineral density (TMD). At weaning, forty female Wistar rats were randomized to 4 different groups and fed “ad libitum”, one diet for the entire period. The diets provided a carbohydrate to fat calorie ratio of 1:1 or 3:1 and a calcium content of 0.6 or 0.3 %: 1) 1:1, 0.6; 2) 1:1, 0.3; 3) 3:1, 0.6; 4) 3:1, 0.3.  At  70 days of age, twenty rats were coupled and at the time of weaning, mothers were evaluated (TM). At days of age: 35, 49 and TM, fat body composition (by chemical method) and tibiae mass density (TMD) by DXA “in vivo” (Lunar DPX) under anesthesia were measured. Results as media and SD were: Diet Day 35 Day 49 TM % body fat TMD (g/cm2) % body fat TMD (g/cm2) % body fat TMD (g/cm2) 1:1-0.6 10.55±1.48 0.82±0.012 11.74±1.69 0.120±0.013 10.72±4.71 0.25±0.03 1:1-0.3 10.40±2.21 0.052±0.005** 11.53±0.99 0.082±0.012** 4.85±2.76* 0.19±0.006 3:1-0.6 8.53±1.48* 0.08±0.006 10.76±1.68 0.11±0.005 3.68±1.21* 0.24±0.4 3:1-0.3 5.56±0.86** 0.06±0.008** 8.8±2.95* 0.07±0.013** 4.92±2.09* 0.21±0.03 ANOVA & Dunnett test. Control group 1:1, 0.6 (*p< 0..5; ** p< 0.01).These results suggest that the replacement of fat by carbohydrate with 50% of calcium in the diet, reduce body fat and TMD at short term. However, TMD remained into normal in TM in response to the mineral content redistribution during lactation. Awarded by UBACyT O-003 y O-004.