Correlation between serum and salivary concentration of biochemical markers of bone turnover

PELLEGRINI GRETEL C; GONZALES CHAVES MACARENA MS; MASTAGLIA SILVINA; FRIEDMAN SILVIA M; ZENI SUSANA N

Nashville, Tennessee. USA

Congreso; Twenty-seventh Annual Meeting of the American Society for Bone and Mineral Research; 2005

American Society for Bone and Mineral Research.

It has been suggested that periodontal disease is a typical early manifestation of osteoporosis; association between them was established in both humans and animals 30 years ago. Proper management of osteoporosis involves early diagnosis of the disease, which includes complementary tests such as assessment of biochemical markers of bone turnover. Currently, remodeling is assessed by serum and urinary biomarkers; however, there is little information concerning presence of these biomarkers in human saliva. The purpose of this study was to determinate whether salivary concentration of bone markers correlated with serum concentration. At the moment of the study 10 young non-pregnant healthy women (aged: 21±2 years) with a self-reported history of medical and dental care were recruited at the school of dentistry, Buenos Aires University, who volunteered for this purpose. In addition, 10 normal adult Wistar rats (300±5g) were studied. Fasting serum (s) and total saliva (sal) were analyzed for bone alkaline phosphatase (b-AL: UL/L) and Carboxy terminal telopeptide of collagen type I (CTX). The b-AL was assessed using a colorimetric method (Wiener Lab) after bone isoenzyme precipitation with wheat-germ lectin, and CTX was measured employing a commercial kit (Crosslaps one step and rat-CTX, respectively. Osteometer Bio Tech, Denmark).Although results were obtained from a small population, data suggest bone markers can be measured in saliva. Significant correlation was observed between saliva and serum concentration of both markers (b-AL: 0.78 and CTX: 0.89). However, whereas sal b-AL was slightly lower (approximately two fold), sal CTX was ten fold lower than that of serum concentration. Although further studies are necessary, this correlation suggests that a simpler and less invasive method exists to assess bone turnover than measurement of serum or urine samples. Since dental visits are more often than medical check-ups, performing routine assay of salivary biomarkers of bone disease in conjunction with delivering routine dental care could allow earlier diagnosis of metabolic bone diseases. Grant M099 UBA. s b-AL Rel s/sal s CTX s b-AL Rel s/sal s CTX Rats 79±12 42±4 1.88±0.28 59.1±16.1 5.8±1.0 10.3±2.7 Women 74±17 49±2 1.71±0.27 32.0± 3.2 2.4±0.1 13.4±1.3