INVESTIGADORES
ZENI Susana Noemi
congresos y reuniones científicas
Título:
Body composition and bone density and its remodelling markers: a follow up study in a rat model for the dietary management of nutritional dwarfing.
Autor/es:
SUAREZ CRISTINA; FERREIRA MONTEIRO ANDREA; RODRIGUEZ PATRICIA; ZENI SUSANA N; FRIEDMAN SILVIA M
Lugar:
San Diego, USA
Reunión:
Congreso; Exp Biology 2005 and the XXXV International Congress of physiological Sciences; 2005
Institución organizadora:
FASEB
Resumen:
Our previous studies demonstrated that the standards of growing rats had similar distribution to those in normal children. Then, in order to study the metabolic alterations in children with nutritional dwarfing (ND), an animal model was prepared. The aim of this work was to obtain reference data of biochemical markers of bone turnover, bone mineral density and body composition, in rats during growth. Wistar female rats (n=20) were fed  an isocaloric diet with a carbohydrate to fat calorie ratio of 1:1 (36% of total energy providing by fat) and a calcium content of 0,6%, from weaning up to an age of 49 days. Body weight was monitored three times a week. At t=21, 28, 35 and 49 the following assessment were performed: 1) Serum Bone Alkaline Phosphatasa (BAP,U/l), 2) Serum Crosslaps (CTX nM, Rat crosslaps.Osteometer Bio Tech), 3) Body Composition (chemical methods, AOAC) and 4) Tibia Bone Mineral Density “ex vivo” (BMD, mg/cm2) (DXA. Lunar DPX). Results:             1:1 0.6 CTX (nM) BAP (U/l) BMD (mg/cm2) % H2O % FAT T=21 375 ± 67 239 ± 22 - 71.95 ± 1.26 8.43 ± 0.82 T=28 582 ± 190* 292 ± 72.96 - 72.26 ± 1.45 8.42 ± 1.55 T=35 362 ± 41 175 ± 85 0.082 ± 0.012 69.96 ± 1.85** 9.97 ± 1.72 T=49 219 ± 116 53 ± 13** 0.120 ± 0.013 67.05 ± 1.45** 10.44 ± 2.33* Shown are Mean ± SD. ANOVA followed by Dunnet test *p<0.01; **p<0.05. Conclusion: These reference data are valuable to design studies to infer and predict the nutritional response in children with ND. Granted by UBACyT O 004, Argentina and Osteometer Bio Tech.