Antitumoral and antioxidant activity of the polyphenol-ich macroalga Cladophora surera

LEZCANO, V.; FERNÁNDEZ, C.; PARODI, E.R.; MORELLI, S.

Nantes

Congreso; 6th Congress of the International Society for Applied Phycology; 2017

ISAP International Society for Applied Phycology

Macroalgae are currently being explored as novel and sustainable sources of bioactive compounds for both pharmaceutical and nutraceutical applications arising from its antioxidant, anticancer and antimicrobial activity. In thepresent study, the antitumoral and antioxidant activities of crude methanolic extract of the freshwater macroalgae Cladophorasurera Parodi&Cáceres, harvested from Napostá Creek in Argentina, were investigated in vitro. The antioxidant activity was assessed by DPPH method and polyphenol content using Folin?Ciocalteu phenol reagent. Antitumoral activity was evaluated on the human breast adenocarcinoma cell line MCF?7 by measuring proliferation, migration and cell adhesion. The algae extract (AE) showed high total phenol content (1.62 ± 0.16 μg GAE / mg dry weight algae) and strong scavenging activity on DPPH radical (2.5 ± 0.2 DPPH% mg dry weight algae).The percentage of alive (unstained) and dead (blue stained) cells was determined by Trypan blue assay. The results indicated that after 48h of treatment the AE significantly inhibits Proliferation in a dose-dependent manner, being more effective the highest doseemployed (80 % ± 7.6 vs. control); with a concomitant increment in dead cells. However, the colorimetric MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay only showed a significant decreasE in cell viability at 100μg/ml AE (20 % ± 3.31 vs. control). Using the wound healing assay we demonstrated that AE inhibits cell migration, with 78.5% and 50.7% of inhibition induced by 10 and 100 μg/ml of AE, espectively. Through a cell adhesion assay we found that AE affects considerably the cell adhesion capacity at all doses probed (78.24 % ±3.6 and 95 % ± 8.36 vs. control, at 10 and 100 μg/ml, respectively). Analysis of cell spreading under microscope indicated that cell morphology was also affected by AE treatment. These results indicate that C. surera could be a source of valuable bioactivecompounds usable as antitumoralpreventive therapy for their effects on the regulation of processes involved in metastasisin cells derived from human mammary cancer (CONICET PIP11220130100070CO; PGI UNS 24/234 and 24/124; PICT 1749)