Cryptic haploid stages in the life cycle of Leathesia marin

POZA, A.M.; SANTIAÑEZ, W.J.E.; CROCE, M.E.; KOGAME, K.; PARODI, E.R.

JOURNAL OF PHYCOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2020 vol. 56 p. 1349 - 1361

0022-3646

We evaluated the life cycle of Leathesia marina through molecular analyses, culture studies, morphological observations, and ploidy measurements. Macroscopic sporophytes were collected from two localities in Atlantic Patagonia and were cultured under long-day (LD) and short-day (SD) conditions. Molecular identification of the microscopic and macroscopic phases was performed through the cox3 and rbcL genes and the phylogeny was assessed on the basis of single gene and concatenated datasets. Nuclear ploidy of each phase was estimated from the DNA contents ofindividual nuclei through epifluorescence microscopy and flow cytometry. Molecular results confirmed the identity of the Argentinian specimens as L. marina and revealed their conspecificity with L. marina from New Zealand, Germany, and Japan. The sporophytic macrothalli (2n) released mitospores from plurilocular sporangia, which developed into globular microthalli (2n), morphologically similar to the sporophytes but not in size, constituting a generation of small diploidthalli, with a mean fluorescent nuclei cross-sectional area of 3.21 0.7 lm2. The unilocular sporangia released meiospores that developed two morphologically different types of microthalli: erect branched microthalli (n) with a nuclear area of 1.48 0.07 μm2 that reproduces asexually, andprostrate branched microthalli (n) with a nuclear area of 1.24 0.10 μm2 that reproduces sexually. The prostrate microthalli released gametes in LD conditions, which merged and produced macroscopic thalli with a nuclear cross-sectional area of 3.45 0.09 μm2. Flow cytometry confirmed that the erect and prostrate microthalli were haploid and that the globular microthalli and macrothalli were diploid.