INVESTIGADORES
PESSINO Silvina Claudia
congresos y reuniones científicas
Título:
In situ expression pattern of a putative MAP kinase gene from Paspalum during ovary development of Brachiaria brizantha.
Autor/es:
DANTAS APA; DUSI D; GUIMARAES LA; PESSINO SC; CARNEIRO V
Lugar:
Brasilia, Brasil
Reunión:
Congreso; XX International Congress on Sexual Plant Reproduction, Brasilia, Brasil; 2008
Institución organizadora:
Plant Reproduction International Society
Resumen:
Plants from the genus Brachiaria are widely cultivated and used as forage for cattle production in the tropical areas of Brazil. The genus Paspalum is mostly found in subtropical areas in the south of Brazil and Argentina. These genera of grasses have plants that reproduce by both, sexuality and apomixis, more specifically by apospory. Because Brachiaria and Paspalum show similarities in their ovule development, we are analyzing the expression of some cDNA sequences found to be of interest for Paspalum apomictic and sexual development in ovaries of Brachiaria. One of them is a cDNA clone N46, sequence corresponding to a MAP kinase (MAP3K) differentially expressed in apomictic and sexual plants of Paspalum notatum. In this work, we examined the pattern of this MAP3K Paspalum gene expression during ovary development in sexual and apomictic Brachiaria brizantha by in situ hybridization. For that, 7 µm sections of ovaries of sexual and apomictic B. brizantha in stages of megasporogenesis and megagametogenesis were used. RNA preservation was observed staining some sections with acridine orange. The sections were hybridized overnight at 42 C with 600 ng ml-1 of sense or antisense digoxigenin-labelled probe in 100 µL of hybridization solution. After post-hybridization treatments the slides were mounted. Signal of hybridization was observed in ovaries, specially in stage of megasporogenesis (archespore or MMC, coenocyte) of sexual and apomictic plants and less intense or absent in later stages. In agreement with prior observations in Paspalum, in the sexual plant the signal was more strong. Unexpectedly, signal was also found when using the sense probe in the same pattern found for the antisense probe. Based on the observed results, we suggest a modulation in gene expression of Paspalum clone 46 during Brachiaria ovary development.