Aqueous micellar two-phase system as an alternative method to selectively remove soy antinutritional factors

HAIDAR, CARLA N.; COSCUETA, EZEQUIEL; CORDISCO, ESTEFANÍA; NERLI, BIBIANA B.; MALPIEDI, LUCIANA P.

LEBENSMITTEL-WISSENSCHAFT UND-TECHNOLOGIE-FOOD SCIENCE AND TECHNOLOGY

ELSEVIER SCIENCE BV

Año: 2018 vol. 93 p. 665 - 672

0023-6438

In this work, different antinutritional factors (trypsin inhibitors, isoflavones and raffinose family oligosaccharides)were selectively removed from soy flour by using aqueous micellar two-phase systems (AMTPS). The effects of independent variables including temperature (30?60 °C), time (10?40 min) and solid to liquid ratio (0.025?0.050 g/L) on the extraction of each antinutritional factor were analyzed using a full factorial design. As general tendency, temperature and time were the most significant parameters (p < 0.05). The best condition forthe selective recovery (97% of isoflavones at top phase, and more than 50% of the rest of ANFs at bottom phase)were 5 g/L of Genapol X-080, 0.2 moL/L of sodium citrate pH 5.00, 30 °C, 40 min and 0.050 g/L. Besides, in vitrogastrointestinal digestions assays demonstrated that the treated soy flour improved its protein digestibility. The findings of this work represent the introduction of a novel methodology to selectively remove soy antinutritionalfactors.