INVESTIGADORES
NERLI Bibiana Beatriz
artículos
Título:
Study of the albumin-polyethyleneglycol interaction to predict the protein partitioning in aqueous two-phase systems.
Autor/es:
FARRUGGIA BEATRIZ; NERLI, BIBIANA; PICÓ GUILLERMO
Revista:
Journal of Chromatography B
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2003 vol. 798 p. 25 - 33
ISSN:
1570-0232
Resumen:
The theoretical framework based only on the excluded volume forces is not enough to explain the bovine serum albumin partitioning behaviour in aqueous biphasic systems. The goal of this work is to look at the phase separation via the polymer effect on the water structure. Our findings suggest that polyethyleneglycol 600-protein interaction is conducted by van derWaals forces between the hydrophobic surfaces from PEG and protein molecules, which implies the rupture of hydrogen bonds from the structured water in their neighbours. Therefore, the protein will concentrate in the most water-structured phase (polyethyleneglycol) in order to reach the minimal free energy condition. When polyethyleneglycol molecular weight increases, its exclusion from protein surface prevails, thus pushing the bovine serum albumin to the bottom phase.