An unknown hydrolase activity of human serum albumin: beta lactamase activity.

NERLI, BIBIANA; GARCÍA, FABIANA; PICÓ, GUILLERMO

Biochemistry and Molecular Biology International

Academic Press Australia

Año: 1995 vol. 37 p. 909 - 915

1039-9712

The interaction of a chromogenic cephalosporin, 3-(2,4 dinitrostyryl)-(6R,7R)-7-(2 thienylacetamido)-ceph-3-em-4-carboxylic acid) (nitrocefin) with human serum albumin was studied. Purified human albumin was showed to have a hydrolase activity, catalyzing the decomposition of the chromogenic cephalosporin. The dimeric and trimeric form of albumin also showed this hydrolase activity. The presence of the following amino acid residues at the catalytic site of albumin was demonstrated: tyrosyls 199 and 411, tryptophan 214, lysyls 195, 225 and 240, histidyl 146, also some argynyls were involucrated.