INVESTIGADORES
NERLI Bibiana Beatriz
artículos
Título:
Porcine pancreatic lipase partition in potassium phosphate-polyethylene glycol aqueous two-phase systems.
Autor/es:
BASSANI GEORGINA; FARRUGGIA BEATRIZ; NERLI, BIBIANA; ROMANINI DIANA; PICÓ GUILLERMO
Revista:
Journal of Chromatography B
Editorial:
Elsevier Science Ireland Ltd.
Referencias:
Año: 2007 vol. 859 p. 222 - 228
ISSN:
1570-0232
Resumen:
This research study examined porcine pancreatic lipase partition in aqueous two-phase systems formed by polyethylene glycol-potassium phosphate at pH 6.0, 7.0 and 8.0, the effect of polymer molecular mass, and NaCl concentration. The enzyme was preferentially partitioned into the polyethylene glycol rich phase in systems with molecular mass 4000?8000, while with polyethylene glycol of 10,000 molecular mass it as concentrated in the phosphate rich phase. The enthalpic and entropic changes found due to the protein partition were negative for all the polyethylene glycol molecular mass systems assessed. Both thermodynamic functions were shown to be associated by an entropic?enthalpic compensation effect suggesting that the water structure ordered in the ethylene chain of polyethylene glycol plays a role in the protein partition. The addition of NaCl increased the lipase affinity to the top phase and this effect was most significant in the system polyethylene glycol 2000?NaCl 3%. This system yielded an enzyme recovery more than 90% with a purification factor of approximately 3.4.