INMIBO ( EX - PROPLAME)   14614
INSTITUTO DE MICOLOGIA Y BOTANICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Lignolityc and cellulolityc activity of strains isolated from compost of agricultural wastes
Autor/es:
BRESSAN FABIANA, GALZENATI VANINA, CARMARÁN CECILIA, CRESPO DIANA
Lugar:
Villa Carlos Paz, Córdoba, Argentina
Reunión:
Congreso; VI Congreso Argentino de Microbiología General; 2009
Institución organizadora:
SAMIGE- Sociedad Argentina de Microbiología General
Resumen:
Cellulolityc and lignolityc activity of fungal strains are involved in the degradation of organic matter. The characterizations of enzymatic profiles from isolated organisms from compost allow identify which ones have a potencial use to develop inoculants and new technologies for improvement compost quality and biodegradation efficiency. In the agricultural waste composting, lignocellulose accounts the major part of biomass and its degradation is essential for the process. The aim of this work was to detect and characterize qualitatively cellulolityc and lignolytic activities of strains isolated from agricultural waste composting (poultry manure + rice hulls + wood thin shaving). Four culture media were used as follows: Carboxy-methyl cellulose agar (CMC) for cellulolytic activity and Tannic acid agar (TA), Galic acid agar (GA) and Guaiacol agar (GuA) for lignolytic activity. During the composting 124 isolates were obtained, of which 17% showed positive reaction for at least one of the media used and only one isolate was positive in all tested media. On the whole of positives, a 86% showed exclusively one enzymatic activity, either cellulolityc or lignolytic; so that 23% was reactive in CMC (mainly strains belonging to the genera Trichoderma and Geotrichum), 38% in GuA and 95% in TA and GA (strains belonging to the genera Alternaria, Epicoccum and Cladosporium). The 14% remaining showed both enzymatic activities and hence have a promissory potential of biodegradation. The most complete profiles were obtained in strains without sporulation (mycelia sterilia). The impact on the technology process is discussed.