PYROSEQUENCING REVEALS CHANGES IN FUNGAL SOIL COMMUNITIES UNDER Lotus tenuis MONOCULTURE

NIEVAS, S; BAILLERES, M; ANA BERNARDINA MENENDEZ; RUIZ, O; LLAMES ME

córdoba

Congreso; LII Reunión de SAIB; 2016

PYROSEQUENCING REVEALS CHANGES IN FUNGAL SOIL COMMUNITIES UNDER Lotus tenuisMONOCULTURENieva AS1, Llames ME1, Bailleres MA2, Menendez AB3, Ruiz OA1.1IIB-INTECH (CONICET/UNSAM). Chascomus, 2EEACuenca del Salado. CEICh. INTA. MAI, 3FCEN. UBA. E-mail: susanna_nieva@hotmail.comFlooding Pampa is the main region for the cattle production in Argentina. The soils from this area are mainlycharacterized chemical and physical limitations. Lotus tenuis is a naturalized forage legume in the Flooding Pampa ableto grow under restrictive conditions, and its use is an alternative to increase the yields in beef production. ―Promotion‖ isan agricultural practice consisting in removing competition from weeds and resident grass using herbicides, so the species of interest grows and fully develops, becoming the plant community into a L. tenuis monoculture grassland.Fungal communities could be affected by the change in the cover grass composition. Through sequencing by 454 FLXRoche technology of the internal transcribed spacer ITS1-ITS2, we studied the structure of fungal communities indifferent soils from flooding pampa, affected by this agronomical practice. Our results suggest that 6-7 years ofapplication of this practice could modify the structure of fungal community evidenced by statistical differences betweenthe structures of fungal community in soils affected by L. tenuis promotion compared with soils under natural grasscover. In addition, indicators reveal the presence of some fungi as the most representative taxa in this soil. Chemicalvariables like conductivity and Sodium are more related to the distribution of different fungi in this environment.