Infection with Micromonospora strain SB3 promotes in vitro growth of Lolium multiflorum plantlets

IANNONE, LEOPOLDO JAVIER; PITTA-ALVAREZ, SANDRA IRENE; NOVAS, MARÍA VICTORIA; NOVAS, MARÍA VICTORIA; SCERVINO, JOSÉ MARTÍN; SCERVINO, JOSÉ MARTÍN; DELLA MÓNICA, IVANA FLORENCIA; QUEREJETA, GISELLE; DELLA MÓNICA, IVANA FLORENCIA; REGALADO GONZÁLEZ, JOSÉ JAVIER; QUEREJETA, GISELLE; REGALADO GONZÁLEZ, JOSÉ JAVIER; IANNONE, LEOPOLDO JAVIER; PITTA-ALVAREZ, SANDRA IRENE

PLANT CELL TISSUE AND ORGAN CULTURE

SPRINGER

Lugar: Berlin; Año: 2018 vol. 134 p. 445 - 455

0167-6857

Cattle breeding is an important economical activity in Argentina, highly dependent ongrass production. In the last decades, grasslands zones were reduced and confined toless productive lands due to the advance of agronomical cultures. Therefore, it isimportant to develop new strategies to improve forage production. New eco-friendlytrends in plant growth promotion include the use of microbial endophytes, but the invitro studies of plant-bioinoculant interactions is limited by the scarce current technological development. In this work, we use a micropropagation protocol for Loliummultiflorum, developed in a previous work, to study the effect of bacterization withactinobacterial endophytes, isolated from Argentine native grasses, on the growth of L.multiflorum in vitro plantlets. To achieve this objective, L. multiflorum plantlets wereinoculated with three Micromonospora strains (SB3, TW2.1 and TW2.2). The resultsobtained showed that the effect of actinobacterial inoculation depends on theMicromonospora strain used. The inoculation with SB3 promoted plant growth,increasing plant biomass, root length and the rate of plantlets ready to be acclimatizedafter four weeks of in vitro culture. Strain TW2.1 did not show, statistically, differencescompared to control treatments, while TW2.2 inhibited plant growth, decreasing plantbiomass, root length and the rate of plants ready to acclimatize. Our results showedthat Micromonospora strain SB3 could be a good candidate to use in breedingprograms for L. multiflorum and other grasses to increase their yield