AFLPcharacterization in pathogenic and coprophilous fungi. Mycotaxon,

CINTO, I. E; GOTTLIEB A M; GALLY, M.; RANALLI M. E.; RAMOS, A. M.

MYCOTAXON

Mycotaxon

Lugar: Ithaca; Año: 2009 vol. 110

0093-4666

Abstract — The objective of this study was to ascertain the usefulness of the AFLP technique in assessing genetic diversity among 47 strains belonging to three Ascomycota genera and as a tool for solving taxonomic problems in related morphological species. Four MseI +1 primers were assayed in combination with two EcoRI +2 and four EcoRI +3 primers. In the present study both +2 and +3 EcoRI primers were informative, but EcoRI +2 produced profiles with high complexity. The addition of the extra selective nucleotide reduced the complexity of the banding patterns generating easily readable patterns to evaluate genetic diversity within and among species. Comparing the results for the three ascomycetous genera used in this study, Colletotrichum (Glomerellecea), Saccobolus (Ascobolaceae) and Iodophanus (Pezizaceae), it could be detected that Colletotrichum presented the highest proportion of polymorphic AFLP loci, followed in order by Iodophanus and Saccobolus. — The objective of this study was to ascertain the usefulness of the AFLP technique in assessing genetic diversity among 47 strains belonging to three Ascomycota genera and as a tool for solving taxonomic problems in related morphological species. Four MseI +1 primers were assayed in combination with two EcoRI +2 and four EcoRI +3 primers. In the present study both +2 and +3 EcoRI primers were informative, but EcoRI +2 produced profiles with high complexity. The addition of the extra selective nucleotide reduced the complexity of the banding patterns generating easily readable patterns to evaluate genetic diversity within and among species. Comparing the results for the three ascomycetous genera used in this study, Colletotrichum (Glomerellecea), Saccobolus (Ascobolaceae) and Iodophanus (Pezizaceae), it could be detected that Colletotrichum presented the highest proportion of polymorphic AFLP loci, followed in order by Iodophanus and Saccobolus. Key words — ascomycetes, genetic characterization, molecular markers, taxonomy — ascomycetes, genetic characterization, molecular markers, taxonomy