INMIBO ( EX - PROPLAME)   14614
INSTITUTO DE MICOLOGIA Y BOTANICA
Unidad Ejecutora - UE
artículos
Título:
Degradation of pruning wastes by Phanerochaete sordidagrowing in SSF. Ultrastructural, chemical and enzymatic studies.
Autor/es:
DIORIO, L., GALATI, B. G., PAPINUTTI, L. V. & AMELA GARCÍA, M. T.
Revista:
International Biodeterioration and Biodegradation
Editorial:
Elsevier
Referencias:
Lugar: Berlin; Año: 2008 vol. 63 p. 19 - 23
ISSN:
0964-8305
Resumen:
A solid standard fermentation (SSF) with the fungus Phanerochaete sordida in a mediumwith Nephrolepiscordifolia (entire pinnae separated from the rachis) and Laurus nobilis (fragmented leaves) was performedover 92 days to study the degradation of leaves with histological, chemical, and enzymatic methods. Thefungus entered the leaves early, through the stomata in N. cordifolia and L. nobilis, and also throughmechanical cuts that had been made in the latter. The initial attack affected the mesophyll in both plantspecies, and the phloem in L. nobilis. The vascular bundle of N. cordifoliawas protected by a sheath of cellswith thick lignified walls. The collenchyma cell walls situated near the principal vein in L. nobilis swelledduring the initial stages of enzymatic action, but reduced their thickness afterwards, mainly in regions ofcontact with the hyphae. At the end of the experiment, no species had leaves with mesophyll. In L. nobilis,phloem was also lacking, and a partial and heterogeneous attack on the xylem became evident. Thehistological changes are compared with the enzymatic activities and the chemical composition of theculture media, describing the stages of fungal colonization.