AFLP molecular characterizations of some Saccobolus species (Ascomycota, Pezizales)

ARACELI M. RAMOS; ISABEL E. CINTO; LUIS FRANCO TADIC; MARÍA E. RANALLI

MYCOTAXON

MYCOTAXON LTD

Año: 2011 vol. 116 p. 355 - 364

0093-4666

Abstract — Amplified restriction fragment length polymorphisms (AFLP) were used toassess the genetic diversity among species and isolates of the genus Saccobolus. Monosporicstrains of five Saccobolus species were used throughout. The dendrogram obtained from theanalysis of grouping (UPGMA) showed four groups of species. The ordination of isolatesthrough the principal coordinates method exhibited nearly the same relations betweenisolates as the phenogram. Analysis of six samples identified as Saccobolus versicolor usingmorphological characters indicated the same diagnostic fingerprints as S. verrucisporuswith the primer combinations tested. The isolates identified as S. versicolor may representan intraspecific variant of S. verrucisporus. The results support the use of AFLP markersto delimit Saccobolus species. This methodology constitutes an additional tool to study thetaxonomy of the genus, which has previously used only morphological characters.