CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MORPHOLOGICAL AND ULTRASTRUCTURAL CHARACTERIZATION OF FIFTH INSTAR NYMPHS HEMOCYTES OF THE HEMATOPHAGOUS INSECT Dipetalogaster maxima (HEMIPTERA: REDUVIIDAE)
Autor/es:
FRUTTERO LL; CANAVOSO LE; MOYETTA NR; LEYRIA J; RAMOS FO
Reunión:
Otro; IV REUNIÓN CONJUNTA DE SOCIEDADES DE BIOLOGÍA DE LA REPÚBLICA ARGENTINA; 2020
Institución organizadora:
Sociedades de Biología de la República Argentina
Resumen:
Hemocytes are cells present in the hemolymph of insects and other invertebrates. These hemolymphatic cells participate in different physiological processes including coagulation and immunity, as well as in the synthesis and transport of nutrients and hormones. The current hemocyte classification, mainly based in morphological characteristics, is a controversial topic particularly in the triatomines, the insect vectors of Chagas disease (Hemiptera: Reduviidae). Several authors have obtained contrasting results, partly because they used different experimental approaches. In this work, we have employed histHemocytes are cells present in the hemolymph of insects and other invertebrates. These hemolymphatic cells participate in different physiological processes including coagulation and immunity, as well as in the synthesis and transport of nutrients and hormones. The current hemocyte classification, mainly based in morphological characteristics, is a controversial topic particularly in the triatomines, the insect vectors of Chagas disease (Hemiptera: Reduviidae). Several authors have obtained contrasting results, partly because they used different experimental approaches. In this work, we have employed histological and cell biology techniques in order to characterize the hemocytes of fifth instar nymphs of the triatomine Dipetalogaster maxima. In addition, it was conducted for the first time an ultrastructural study by transmission electron microscopy. Using fresh preparations and contrast phase microscopy, the methodology with most consensus in the literature, were identified six populations of hemocytes: plasmatocytes, granulocytes, prohemocytes, oenocytes, adipocytes and giant cells, being the first two the most abundant. These cellular populations were also characterized by immunofluorescence by the employment of an anti-tubulin antibody and by transmission electron microscopy. All cell populations presented a high variation in morphology and size, explaining in part the scarce coincidence among the previously reported finding of diverse authors. This work provides novel information regarding a complex aspect of physiology and cell biology of triatomines and establishes the foundations for future studies directed to understand the specific roles of such hemocyte populations in the defense mechanism of insects.