CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Identification of glomerular biomarkers in urine cells from patients with type 2 diabetes
Autor/es:
MAZZOCCO, YANINA LUCIANA; PONTE, M. GABRIELA; RUIZ PECCHIO, A; EBERHARDT, NATALIA; MEUNIER, EDITH G; VERGOTTINI ANA S; BERGERO, GASTÓN; AOKI, MARIA PILAR
Reunión:
Congreso; Reunión Anual de Sociedades de Biociencias 2020; 2020
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Type 2 Diabetes Mellitus (T2DM) is characterized by the presence of insulin resistance and a prolonged asymptomatic period that makes early diagnosis difficult, so the identification of early damage markers such as those associated with diabetic nephropathy (DN) is extremely valuable. In this context, the involvement of CD39 and CD73 ectoenzymes in DN is not entirely clear, but many studies suggest that alterations in the extracellular ATP metabolic machinery trigger the pathophysiology of the disease. Our objective was to identify early markers of glomerular kidney damage and the expression of CD39 and CD73 in urine cells of T2DM patients. To this aim, the expressions of nephrin (NEF), podocalyxin (PDX), CD39 and CD73 were evaluated by immunofluorescence (IF) in renal explants and in the first morning urine of T2DM patients to determine the detachment of podocytes. In addition, the plasma profile of cytokines was analyzed by LengendPlex and flow cytometry. The IF revealed that PDX is an exclusive marker for podocytes, while NEF was widely expressed in tubules and Bowman´s capsule cells. CD39 was detected in vasculature cells and CD73 was located ubiquitously in the kidney. Preliminary results showed that T2DM patients presented about 2 fold and 3 fold higher frequency of urine NEF+ and PDX+ cells, respectively, than control donors. Furthermore, the percentage of NEF+ and PDX+ cells expressing CD39 in the urine of T2DM patients were about 5 times higher than the corresponding cells from control donors. Finally, the median fluorescence intensity (MFI) of IL-6 (101,92±44,13), IL-4 (130,65±50,18) and TNF-a (130,65±30,14) in plasma from T2DM patients (n=4) tend to be higher that the control counterparts (n=5) (IL-6 69,26±9,26 (p= 0.1435); IL-4 93,91±7,43 (p= 0.3819); and TNF-a 46,49±0,69 (p= 0.0824)). In conclusion, the results suggest that urine from T2DM patients presented increased detached podocytes, which expressed higher levels of purinergic pathway ectoenzymes.