Early Treg cell depletion during trypanosoma cruzi infection promotes CD8+ T cell immunity and parasite control in the acute and chronic phases

BOCCARDO S; GRUPPI A; ARAUJO FURLAN CL; MONTES CL; RODRIGUEZ C; ACOSTA RODRÍGUEZ EV

Buenos Aires

Congreso; Reunión Anual de Sociedades de Biociencias - SAIC. SAI. SAFIS. 2020; 2020

SAIC. SAI. SAFIS.

We reported that after Trypanosoma cruzi (Tc) infection, Tregs undergoa marked and sustained reduction in frequency. This naturalcontraction of the Treg response was critical to allow the emergenceof protective anti-parasite CD8+ T cell immunity in the acute phase.Accordingly, we previously demonstrated that Treg depletion at day(d) 5 post-infection (pi) but not at d11pi impacted on the magnitudeof anti-parasite CD8+ T cell response and the ability to control parasitereplication in the acute phase. Considering this, we hypothesizedthat Tregs may exert a role during early events of T cell priming.To test this, DEREG mice were infected with Tc and injected withdiphtheria toxin (DT) or PBS at d5 and 6pi. The next day, DT-treatedanimals showed increased numbers of B cells, monocytes and neutrophilsin the spleen compared to controls (p