CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A transcriptomic approach to understand the role of tumor-infiltrating CD39+ CD4+ FOXP3neg T cells.
Autor/es:
BOSSIO, SABRINA N.; BOARI, JIMENA TOSELLO; GRUPPI, ADRIANA; CONSTANZA RODRIGUEZ; ELMER FERNÁNDEZ; MONTES, CAROLINA L.; ABRATE, CAROLINA; PIAGGIO, ELIANE; RODRÍGUEZ, EVA V. ACOSTA
Reunión:
Congreso; Reunión Anual de Sociedades de Biociencias. SAIC - SAI - SAFIS; 2020
Resumen:
The accumulation of tumor-infiltrating (TI) CD8+ T cells plays a pivotal role in the immune response against tumors. However, the function of CD4+ T cells within Tumor microenvironment (TME) remains uncertain. We previously demonstrated that tumors from tumor-bearing mice showed an important infiltrate of FOXP3neg CD4+ cells (Tconv) expressing CD39. CD39 is an ecto-enzyme involved in the conversion of eATP to immunomodulatory adenosine. We aim to determine the transcriptional profile of TI-CD39+Tconv cells. FOXP3-GFP mice (N=3), were injected with B16F10-OVA cancer cells. On day 17 the following TI-CD4+T cells were isolated by FACS: CD39+Tconv, CD39-Tconv and Treg. Then, we perform the transcriptome sequencing by RNAseq. Principal component analysis showed that all populations analyzed clustered separately, reflecting their differential transcriptional profile. To define the molecular profile of CD39+Tconv cells, we searched for differentially expressed genes (DEGs). We found 449 and 153 significantly up and down-regulated DEGs (p.adj