NANOPARTICULATE-BASED VACCINE PROTECTS AGAINST Fasciola hepática INFECTION THROUGH IL-17 AND ANTIBODIES DEPENDENT MECHANISMS.

SILVANE L, CELIAS D, MALETTO B, ROMAGNOLI P, CHIAPELLO L, PALMA S, ALLEMANDI D, SANABRIA R, PRUZZO C, MOTRAN C, CERVI L.

Tucuman

Congreso; LXVII REUNIÓN SAI; 2019

123. NANOPARTICULATE-BASED VACCINE PROTECTS AGAINST Fasciola hepaticaINFECTION THROUGH IL-17 AND ANTIBODIES DEPENDENT MECHANISMSSilvane L1,2, Celias D1,2, Maletto B1,2, Romagnoli P3, Chiapello L1,2, Palma S5, Allemandi D5,Sanabria R4, Pruzzo C4, Motran C1,2, Cervi L1,2.1Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, UNC, Córdoba, Argentina.2CIBICI, CONICET-UNC. 3IUCBC, Córdoba, Argentina. 4IIB-INTECH, CONICET-UNSAM. UNLP, LaPlata, Argentina. 5UNITEFA, CONICET-UNC.F. hepatica is a helminth that causes a chronic disease mainly in cattle, sheep and occasionally inhumans. The limitations of chemotherapy have led to the development of vaccines to protectagainst F. hepatica infection. Kunitz type molecule (Fh-KTM) is a highly expressed protein in theparasite and plays a vital role in its survival.In this work we evaluate the mechanisms by which Fh-KTM formulated with a liquid crystalnanostructure (CpG-ODN/Coa-ASC16) induces protection against this parasite.BALB/c mice were weekly immunized for 3 weeks with Fh-KTM/CpG-ODN/Coa-ASC16, CpGODN/Coa-ASC16 alone or saline. One week after last immunization the mice were orallychallenge with F. hepatica metacercariae.The vaccinated mice group showed significantly improved survival rates (p> 0.05 Mantel-CoxTest) compared with CpG-ODN/Coa-ASC16 immunized and the infected control. In correlation,liver damage and transaminase enzymes exhibited reduced levels in vaccinated mice. Besides,Fh-KTM/CpG-ODN/Coa-ASC16 vaccinated mice also showed high levels of specific IgG1, IgG2aand IgA in serum (p>0.05 ANOVA) compared with another groups. In addition, IgA titers wereincreased in intestinal lavage and feces (p>0.05 ANOVA). Systemic IL-17 and IFN-γ cytokineslevels were higher in vaccinated mice (p>0.05 ANOVA) than in the other groups.The neutralizing treatment of IL-17 in vaccinated mice decreased IgG2a and IgA antibody levelsas well as IFN-γ production (p>0.05 ANOVA). Remarkably, the IL-17 neutralization led to anincrease in the mortality of vaccinated mice.These results indicate that IL-17 production is a key event for the protective mechanismsagainst F. hepatica through the IFN-γ and antibodies secretion.