MINTHOSTACHYS VERTICILLATA ESSENTIAL OIL MICROCAPSULE AS POSSIBLE CANDIDATE FOR VACCINE ADJUVANT

CAMPRA NA, MONTIRONI I, MOLIVA M, ARSAUTE S, RAVIOLO J, CAMACHO N, PAREDES A, MALETTO B, PALMA S, REINOSO EB, CARIDDI, LN

Tucuman

Congreso; LXVII REUNIÓN SAI; 2019

75. MINTHOSTACHYS VERTICILLATA ESSENTIAL OIL MICROCAPSULE AS POSSIBLECANDIDATE FOR VACCINE ADJUVANTCampra NA1, Montironi I1, Moliva M1, Arsaute S1, Raviolo J2, Camacho N3, Paredes A3, Maletto B3,Palma S3, Reinoso EB1, Cariddi LN1.1INBIAS-Universidad Nacional de Río Cuarto. 2Facultad de Agronomía y Veterinaria, UniversidadNacional de Río Cuarto. 3Universidad Nacional de Córdoba.In a previous study we demonstrated that Minthostachys verticillata essential oil (2.5 mg/ml)has adjuvant effect using OVA as antigen. The aim of this study was to determine the effect of EOmicrocapsule (EOMC) against humoral and cellular immune response using Enterococcusfaecium as antigen. Balb/c mice (n=4 per group) were immunized by subcutaneous injection(day 0) and revaccinated (day 14 and 28) as follows: Group 1:100 μl saline solution; Group2:100 μl E. faecium bacterin 6x109 CFU/ml; Group 3: 100 μl bacterin + Incomplete Freund´sAdjuvant 50%; Group 4: 100 μl bacterin +EO (2,5 mg/ml); Group 5: 100 μl bacterin +microcapsule wall material (2,5 mg/ml); Group 6: 100 μl bacterin + EOMC (5 mg/ml). Sevendays after last revaccination, serum samples were collected and analyzed for antigen-specifictotal antibodies (IgG, IgM and IgA) by indirect ELISA. In addition, splenic lymphocytes wereobtained for XTT proliferation assay and quantification of CD4+ and CD8+ T cells by flowcytometer. Any mouse died; however only in group 3 a significant induration at the injectionsite was observed. EOMC induced the highest antibodies levels (p