A nanostructure of ascorbyl palmitate used as vaccine platform improv

NICOLAS DHO; SANTIAGO PALMA; BELKYS MALETTO; CONSTANZA MARIN; DANIEL ALBERTO ALLEMANDI; GABRIEL MORON; FEDERICO RUIZ MORENO; MARIA MERCEDES PASCUAL; MARIA CRISTINA PISTORESI-PALENCIA.

virtual

Congreso; LXVIII REUNIÓN SAI; 2020

Previously, we demonstrated that the nanoformulation of OVA and CpG-ODN with a nanostructure formed by self-assembly of 6-O-ascorbyl palmitate (Coa-ASC16) elicited immune response superior to those induced by the soluble counterpart. Here, we studied the effects of various formulations of vaccine components on antigen-specific memory response and on antigen persistence at injection site. Mice were subcutaneously immunized with a single-dose of OVA and CpG-ODN nanoformulated with Coa-ASC16 (OCC), with OVA and CpG-ODN in solution (OC) prepared to room temperature (RT), with OVA and CpG-ODN solution heated and then cooled down to RT (OCø), with OVA solution heated and then cooled down to RT plus CpG-ODN solution at RT (Oø/C), with OVA solution at RT plus CpG-ODN solution heated and then cooled down to RT (O/Cø). Heating and cooling processes recreated the conditions of the nanoformulation preparation. On the 160th day of post-immunization (pi), mice were intraperitoneally challenged with OVA/CpG-ODN and sacrificed 7 days later. OVA-anti IgG titers (ELISA) and splenocytes by flow cytometry were evaluated. When antigens at the injection site were measured, the formulations were prepared using fluorescent-dye labeled OVA and in vivo scanning of mice was performed on Odyssey®CLx. OCC induced IgG titers higher than OCø and Oø/C at 145 day pi (p