CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Metabolites of the Kyn pathway participate in the control of the T. cruzi infection in human placental explants
Autor/es:
THEUMER, MG; PIEGARI, M; FRETES, RE; MEZZANO, L; CUELLO, L; DÍAZ.LUJÁN, CM; MOREIRA-ESPINOZA, MJ; BENIZIO, E; TRIQUELL, MF
Lugar:
CABA
Reunión:
Congreso; International Federation of Placenta Associations; 2019
Institución organizadora:
International Federation of Placenta Associations
Resumen:
Objective: to evaluate the effect of the kynurenine (Kyn) pathway (KP) metabolites at controlling the infection by Trypanosoma cruzi in human placental explants. Methods: Human placental explants were cultured with or without T. cruzi (105/ml trypomastigotes, tulahuen strain) for 24 h. Models received drugs treatments or not (control). The first model used L-1-Metyl-tryptophan (L-1MT, 400 µM) and Interferon-ɣ (IFN-ɣ, 20 ng/mL) for 72 h. The second one, L-Tryptophan (L-Trp, 200 µM), and the last one used 3-hidroxykynurenine or 3-hidroxyantranilic acid metabolites of the KP (3-HK, 100 µM or 3-HAA, 100 µM respectively) each for 24 h until reaching 96 h. Trp was measured by HPLC. Kyn production and specific IDO activity were measured by colorimetric assay in culture supernatants and homogenate of explants. Parasitic load was quantified by real time PCR for T. cruzi. GraphPad Prism7.0 software was used for statistical analysis of data. ANOVA or Student?s t-test were used to compare treated explants with the controls. Significance was set at p-value