CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
NANOPARTICULATE-BASED VACCINE PROTECTS AGAINST Fasciola hepatica INFECTION THROUGH IL-17 AND ANTIBODIES DEPENDENT MECHANISMS
Autor/es:
CELIAS, D; CHIAPELLO, LAURA SILVINA; SANABRIA, R; CERVI, LAP; MALETTO, BELKYS; PALMA, SANTIAGO; PRUZZO, C; SILVANE, LEONARDO; ROMAGNOLI, PABLO; ALLEMANDI, DANIEL; CLAUDIA MOTRAN
Lugar:
Tucuman
Reunión:
Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología (SAI). San Miguel de Tucumán; 2019
Institución organizadora:
SAI
Resumen:
F. hepatica is a helminth that causes a chronic disease mainly in cattle, sheep and occasionally inhumans. The limitations of chemotherapy have led to the development of vaccines to protectagainst F. hepatica infection. Kunitz type molecule (Fh-KTM) is a highly expressed protein in theparasite and plays a vital role in its survival.In this work we evaluate the mechanisms by which Fh-KTM formulated with a liquid crystalnanostructure (CpG-ODN/Coa-ASC16) induces protection against this parasite.BALB/c mice were weekly immunized for 3 weeks with Fh-KTM/CpG-ODN/Coa-ASC16, CpGODN/Coa-ASC16 alone or saline. One week after last immunization the mice were orallychallenge with F. hepatica metacercariae.The vaccinated mice group showed significantly improved survival rates (p> 0.05 Mantel-CoxTest) compared with CpG-ODN/Coa-ASC16 immunized and the infected control. In correlation,liver damage and transaminase enzymes exhibited reduced levels in vaccinated mice. Besides,Fh-KTM/CpG-ODN/Coa-ASC16 vaccinated mice also showed high levels of specific IgG1, IgG2aand IgA in serum (p>0.05 ANOVA) compared with another groups. In addition, IgA titers wereincreased in intestinal lavage and feces (p>0.05 ANOVA). Systemic IL-17 and IFN-γ cytokineslevels were higher in vaccinated mice (p>0.05 ANOVA) than in the other groups.The neutralizing treatment of IL-17 in vaccinated mice decreased IgG2a and IgA antibody levelsas well as IFN-γ production (p>0.05 ANOVA). Remarkably, the IL-17 neutralization led to anincrease in the mortality of vaccinated mice.These results indicate that IL-17 production is a key event for the protective mechanismsagainst F. hepatica through the IFN-γ and antibodies secretion.