CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Lsp1-/- DENDRITIC CELLS INDUCE POOR PROLIFERATION OF CD4+ T LYMPHOCYTES
Autor/es:
PASCUAL, MARÍA MERCEDES; PISTORESI, MARÍA CRISTINA; CRESPO, MARÍA INÉS; MALETTO, BELKYS; DHO, NICOLÁS DANIEL; MARÍN, CONSTANZA; MORÓN, GABRIEL
Lugar:
Tucuman
Reunión:
Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología; 2019
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Leukocyte-specific protein 1 (LSP1) is a 52 kDa cytoplasmic F-actin binding phosphoprotein expressed in all human and murine leukocytes and endothelial cells. LSP1 is an important regulator of actin cytoskeleton remodelling. We have previously shown that Lsp1-/- mice have an impaired CTL response after antigen exposure, with Lsp1-/- dendritic cells (DCs) failing to induce a strong CTL response in vivo.In order to study the role of LSP1 in DCs to promote a CD4+ T cell-mediated response, we first evaluated the capacity of Lsp1-/- DCs to activate CD4+ T cells. In vitro, bone marrow dendritic cells (BMDCs) were derived from Lsp1-/- mice with Flt3-L. BMDCs were pulsed with soluble or particulated Ovalbumin (OVAw or OVAb, respectively), then stimulated with CpG-ODN 1826 and finally cocultured with CD4+ T cells purified from OT-II mice, previously stained with e-Fluor 670 proliferation dye. Three days later, a significantly lower percentage of proliferating cells was observed with Lsp1-/- BMDCs incubated with OVAw (p< 0.0001) and OVAb (p< 0.0001), and CD25 expression with Lsp1-/- BMDCs incubated with OVAb (p< 0.005) compared to Lsp1+/+ BMDCs. In vivo, CD4+ T cells from OT-II mice were equally stained and injected into Lsp1-/- mice. Twenty-four hours later, mice were immunized with OVAw+CpG-ODN. Three days later, we observed a significantly lower frequency of proliferating CD4+ T cell in draining lymph nodes (p< 0.05) in Lsp1-/- mice compared to Lsp1+/+ mice.These results suggest that LSP1 deficiency affects CD4+ T cell activation, w