Genomic analysis of vitamin E QTL in tomato fruit

J. ALMEIDA, L. QUADRANA, R. ASÍS, F. DE GODOY, F. CARRARI, M. ROSSI.

Dundee, Scotland

Conferencia; 7th Solanaceae conference; 2010

Genomic analysis of vitamin E QTL in tomato fruit J. Almeida1, L. Quadrana2, R. Asís3, F. de Godoy1, F. Carrari2, M. Rossi1.1, L. Quadrana2, R. Asís3, F. de Godoy1, F. Carrari2, M. Rossi1. 1Departamento de Botânica‐IB‐USP, 277, 05508‐900, São Paulo, SP, Brazil.Departamento de Botânica‐IB‐USP, 277, 05508‐900, São Paulo, SP, Brazil. 2Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaría (IB‐INTA), and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), PO Box 25, B1712WAA Castelar, Argentina (partner group of the Max Planck Institute for Molecular Plant Physiology, Potsdam‐Golm, Germany).Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaría (IB‐INTA), and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), PO Box 25, B1712WAA Castelar, Argentina (partner group of the Max Planck Institute for Molecular Plant Physiology, Potsdam‐Golm, Germany).‐Golm, Germany). 3CIBICI, Facultad de Ciencias Químicas Universidad Nacional de Córdoba, CC 5000, Córdoba, Argentina.CIBICI, Facultad de Ciencias Químicas Universidad Nacional de Córdoba, CC 5000, Córdoba, Argentina. juliana_abs@yahoo.com.br Vitamin E, which is naturally found in tomato (S. lycopersicum), plays an essential role as antioxidant for scavenging ROS generated from cellular processes. Understanding the mechanisms underlying synthesis, transport and accumulation of vitamin E in crops are of great interest because of its implications for human health. In the present work, a fruit tocopherol profile was evaluated in S. pennellii introgression lines that either presented vitamin E QTL previously described, or that their introgression fragment spanned genes for the biosynthesis core pathway. Several quantitative trait loci (QTL) for vitamin E were found, some of those showed to be stable over different harvest years when compared against previously published data. A candidate gene approach allowed the identification of twelve genes co‐locating with these QTL. The cDNA from the wild alleles were cloned and sequenced and the comparative analysis revealed polymorphisms in nucleotides and deduced amino acids sequences between S. lycopersicum and S. pennellii alleles. These results represent an important step for understanding the genetic determinants of vitamin E natural variation in tomato fruit.S. lycopersicum), plays an essential role as antioxidant for scavenging ROS generated from cellular processes. Understanding the mechanisms underlying synthesis, transport and accumulation of vitamin E in crops are of great interest because of its implications for human health. In the present work, a fruit tocopherol profile was evaluated in S. pennellii introgression lines that either presented vitamin E QTL previously described, or that their introgression fragment spanned genes for the biosynthesis core pathway. Several quantitative trait loci (QTL) for vitamin E were found, some of those showed to be stable over different harvest years when compared against previously published data. A candidate gene approach allowed the identification of twelve genes co‐locating with these QTL. The cDNA from the wild alleles were cloned and sequenced and the comparative analysis revealed polymorphisms in nucleotides and deduced amino acids sequences between S. lycopersicum and S. pennellii alleles. These results represent an important step for understanding the genetic determinants of vitamin E natural variation in tomato fruit.S. pennellii introgression lines that either presented vitamin E QTL previously described, or that their introgression fragment spanned genes for the biosynthesis core pathway. Several quantitative trait loci (QTL) for vitamin E were found, some of those showed to be stable over different harvest years when compared against previously published data. A candidate gene approach allowed the identification of twelve genes co‐locating with these QTL. The cDNA from the wild alleles were cloned and sequenced and the comparative analysis revealed polymorphisms in nucleotides and deduced amino acids sequences between S. lycopersicum and S. pennellii alleles. These results represent an important step for understanding the genetic determinants of vitamin E natural variation in tomato fruit.introgression lines that either presented vitamin E QTL previously described, or that their introgression fragment spanned genes for the biosynthesis core pathway. Several quantitative trait loci (QTL) for vitamin E were found, some of those showed to be stable over different harvest years when compared against previously published data. A candidate gene approach allowed the identification of twelve genes co‐locating with these QTL. The cDNA from the wild alleles were cloned and sequenced and the comparative analysis revealed polymorphisms in nucleotides and deduced amino acids sequences between S. lycopersicum and S. pennellii alleles. These results represent an important step for understanding the genetic determinants of vitamin E natural variation in tomato fruit.‐locating with these QTL. The cDNA from the wild alleles were cloned and sequenced and the comparative analysis revealed polymorphisms in nucleotides and deduced amino acids sequences between S. lycopersicum and S. pennellii alleles. These results represent an important step for understanding the genetic determinants of vitamin E natural variation in tomato fruit.S. lycopersicum and S. pennellii alleles. These results represent an important step for understanding the genetic determinants of vitamin E natural variation in tomato fruit.