CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Functional analysis of vitamin E biosynthesis in tomato fruits by a gfp-based VIGS system.
Autor/es:
LEANDRO QUADRANA, RAMON ASIS, MARIANA LOPEZ, JULIANA ALMEIDA, ALISDAIR R. FERNIE, MAGDALENA ROSSI, FERNANDO CARRARI
Lugar:
Dundee, Escocia
Reunión:
Conferencia; 7th Solanaceae conference; 2010
Resumen:
Functional analysis of vitamin E biosynthesis in tomato fruits by a gfp‐based VIGS system‐based VIGS system
Leandro Quadrana1, Ramon Asis3, Mariana Lopez1, Juliana Almeida2, Alisdair R. Fernie4, Magdalena
Rossi2, Fernando Carrari1.
Rossi2, Fernando Carrari1.
1, Ramon Asis3, Mariana Lopez1, Juliana Almeida2, Alisdair R. Fernie4, Magdalena
Rossi2, Fernando Carrari1.2, Fernando Carrari1.
1Instituto de Biotecnologia, Instituto Nacional de Tecnologia Agropecuaria (IB‐INTA), and Consejo Nacional de
Investigaciones Científicas y Técnicas (CONICET), PO Box 25, B1712WAA Castelar, Argentina (partner group of
the Max Planck Institute for Molecular Plant Physiology, Potsdam‐Golm, Germany).
Investigaciones Científicas y Técnicas (CONICET), PO Box 25, B1712WAA Castelar, Argentina (partner group of
the Max Planck Institute for Molecular Plant Physiology, Potsdam‐Golm, Germany).
Instituto de Biotecnologia, Instituto Nacional de Tecnologia Agropecuaria (IB‐INTA), and Consejo Nacional de
Investigaciones Científicas y Técnicas (CONICET), PO Box 25, B1712WAA Castelar, Argentina (partner group of
the Max Planck Institute for Molecular Plant Physiology, Potsdam‐Golm, Germany).‐Golm, Germany).
2Departamento de Botanica‐IB‐USP, 277, 05508‐900, São Paulo, SP, Brazil.Departamento de Botanica‐IB‐USP, 277, 05508‐900, São Paulo, SP, Brazil.
3CIBICI, Facultad de Ciencias Quimicas Universidad Nacional de Cordoba, CC 5000, Cordoba, Argentina.CIBICI, Facultad de Ciencias Quimicas Universidad Nacional de Cordoba, CC 5000, Cordoba, Argentina.
4Max Planck Institute for Molecular Plant Physiology, Golm, GermanyMax Planck Institute for Molecular Plant Physiology, Golm, Germany
lquadrana@cnia.inta.gov.ar
Vitamin E comprises a family of amphiphilic antioxidants incluiding tocopherols and tocotrienols,
which are exclusively synthesized by photosynthetic organisms. Four isomers: alfa, beta, gamma
and delta tocopherol are produced in tomato. Alfa‐ tocopherol is the most abundant in fruits and
has the mayor vitamin E related activity. Even when most of the enzymes of Vitamin E biosynthesis
were characterised in Arabidopis, the understanding of this biosynthetic pathway in tomato is
scarce.
By screening the Solanum pennellii introgression line (IL) population we found that ripe fruits from IL
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
scarce.
By screening the Solanum pennellii introgression line (IL) population we found that ripe fruits from IL
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
has the mayor vitamin E related activity. Even when most of the enzymes of Vitamin E biosynthesis
were characterised in Arabidopis, the understanding of this biosynthetic pathway in tomato is
scarce.
By screening the Solanum pennellii introgression line (IL) population we found that ripe fruits from IL
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
scarce.
By screening the Solanum pennellii introgression line (IL) population we found that ripe fruits from IL
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
‐ tocopherol is the most abundant in fruits and
has the mayor vitamin E related activity. Even when most of the enzymes of Vitamin E biosynthesis
were characterised in Arabidopis, the understanding of this biosynthetic pathway in tomato is
scarce.
By screening the Solanum pennellii introgression line (IL) population we found that ripe fruits from IL
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
scarce.
By screening the Solanum pennellii introgression line (IL) population we found that ripe fruits from IL
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
Arabidopis, the understanding of this biosynthetic pathway in tomato is
scarce.
By screening the Solanum pennellii introgression line (IL) population we found that ripe fruits from IL
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
Solanum pennellii introgression line (IL) population we found that ripe fruits from IL
8‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
‐2, 8‐2‐1, 9‐2 and 9‐2‐6 present significant alterations in their tocopherol profiles. Moreover,
genomic analyses allowed identification of slvte1, slvte4 and slvte3, orthologs to the corresponding
Arabidopsis genes onto the regions spanning the mentioned introgressions.
In order to study the function of these genes in tomato, we designed a suitable platform for
functional studies based on viral induced gene silencing (VIGS). To overcome the well known
limitations imposed by this system (ie. patchy phenotypes, pleiotropic effects of the tracking
marker), we first analysed the impact on fruit metabolism of two reporter genes, the phytoene
desaturase (pds) and gfp in WT and transgenic GFP plants respectively. GC‐MS metabolite profiles
demonstrated that pds silencing produces dramatic alterations on pigments contents (carotenoids
and tocopherols) in both mature green and ripe tomato fruits. By contrast, gfp silencing produces
alterations in neither primary metabolism nor pigments accumulation, making it a suitable system
for tocopherol metabolism studies. Results obtained by silencing tocopherol genes in fruits will be
discussed.
demonstrated that pds silencing produces dramatic alterations on pigments contents (carotenoids
and tocopherols) in both mature green and ripe tomato fruits. By contrast, gfp silencing produces
alterations in neither primary metabolism nor pigments accumulation, making it a suitable system
for tocopherol metabolism studies. Results obtained by silencing tocopherol genes in fruits will be
discussed.
In order to study the function of these genes in tomato, we designed a suitable platform for
functional studies based on viral induced gene silencing (VIGS). To overcome the well known
limitations imposed by this system (ie. patchy phenotypes, pleiotropic effects of the tracking
marker), we first analysed the impact on fruit metabolism of two reporter genes, the phytoene
desaturase (pds) and gfp in WT and transgenic GFP plants respectively. GC‐MS metabolite profiles
demonstrated that pds silencing produces dramatic alterations on pigments contents (carotenoids
and tocopherols) in both mature green and ripe tomato fruits. By contrast, gfp silencing produces
alterations in neither primary metabolism nor pigments accumulation, making it a suitable system
for tocopherol metabolism studies. Results obtained by silencing tocopherol genes in fruits will be
discussed.
demonstrated that pds silencing produces dramatic alterations on pigments contents (carotenoids
and tocopherols) in both mature green and ripe tomato fruits. By contrast, gfp silencing produces
alterations in neither primary metabolism nor pigments accumulation, making it a suitable system
for tocopherol metabolism studies. Results obtained by silencing tocopherol genes in fruits will be
discussed.
genes onto the regions spanning the mentioned introgressions.
In order to study the function of these genes in tomato, we designed a suitable platform for
functional studies based on viral induced gene silencing (VIGS). To overcome the well known
limitations imposed by this system (ie. patchy phenotypes, pleiotropic effects of the tracking
marker), we first analysed the impact on fruit metabolism of two reporter genes, the phytoene
desaturase (pds) and gfp in WT and transgenic GFP plants respectively. GC‐MS metabolite profiles
demonstrated that pds silencing produces dramatic alterations on pigments contents (carotenoids
and tocopherols) in both mature green and ripe tomato fruits. By contrast, gfp silencing produces
alterations in neither primary metabolism nor pigments accumulation, making it a suitable system
for tocopherol metabolism studies. Results obtained by silencing tocopherol genes in fruits will be
discussed.
demonstrated that pds silencing produces dramatic alterations on pigments contents (carotenoids
and tocopherols) in both mature green and ripe tomato fruits. By contrast, gfp silencing produces
alterations in neither primary metabolism nor pigments accumulation, making it a suitable system
for tocopherol metabolism studies. Results obtained by silencing tocopherol genes in fruits will be
discussed.
‐MS metabolite profiles
demonstrated that pds silencing produces dramatic alterations on pigments contents (carotenoids
and tocopherols) in both mature green and ripe tomato fruits. By contrast, gfp silencing produces
alterations in neither primary metabolism nor pigments accumulation, making it a suitable system
for tocopherol metabolism studies. Results obtained by silencing tocopherol genes in fruits will be
discussed.