CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
C. ALBICANS BETA-1,3-GLUCAN ACTIVATES TYPE I INTERFERONS PATHWAY IN EPITHELIAL CELLS OF THE FEMALE GENITAL TRACT WHICH MODULATES THE LOCAL IMMUNE RESPONSE IN VULVOVAGINAL CANDIDIASIS
Autor/es:
ANGIOLINI, SOFÍA CARLA; ICELY, PAULA ALEJANDRA; ANGIOLINI, SOFÍA CARLA; ICELY, PAULA ALEJANDRA; VIGEZZI, CECILIA; SOTOMAYOR, CLAUDIA ELENA; VIGEZZI, CECILIA; SOTOMAYOR, CLAUDIA ELENA; RODRIGUEZ, EMILSE; MIRÓ, MARÍA SOLEDAD; RODRIGUEZ, EMILSE; MIRÓ, MARÍA SOLEDAD
Lugar:
Tucumán
Reunión:
Congreso; LXVII Reunion Anual de a Sociedad Argentina de Inmunología; 2019
Resumen:
Type I interferons(IFNs-I) function in fungal pathogenesis is little known. C. albicans (Ca) is causal agent of Vulvovaginal Candidiasis(VVC), an inflammatory disease affecting 75% of women worldwide. The aim was to study the fungal elements involved in IFNs-I pathway activation in epithelial cells of female genital tract (FGT) and its ability to modulate the local immune response during VVC development. Two experimental strategies were used: a) In vitro cultured of FGT cell and b) VVC murine model. Human cervical epithelial cells(HeLa) were stimulated with: viable Ca-SC5314(5:1 ratio); Ca-Heat-Killed; Ca-FKS1.S645P (-glucan defective strain); Curdlan (Dectin-1 agonist) and LPS(control) during 4h. IFNβ, IRF3 and IRF7 mRNA levels were measured by qPCR. Our results showed that yeast-to-hypha switch of Ca and the exposure of β-glucan in the fungal wall were able to activate IFNs-I pathway in epithelial cells showing strong induction of IFNβ and IRF7 mRNA (p