CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of Dectin-1/beta-glucan innate immune recognition during Candida albicans systemic infection.
Autor/es:
VIGEZZI, CECILIA; MIRÓ, MARÍA SOLEDAD; SOTOMAYOR, CLAUDIA ELENA; ICELY, PAULA ALEJANDRA; CAEIRO, JUAN PABLO; RODRIGUEZ, EMILSE; RIERA, FERNANDO OSCAR
Reunión:
Congreso; 17th INFOCUS- 1ST ISHAM Latin American Congress; 2019
Resumen:
Invasive candidiasis is an important fungal infection associated with medical care.It is widely recognized as one of the main causes of morbidity and mortality in the healthcare environment, representing a great socio-economic impact on public health. This pathology can be caused by several Candida species, being C.albicans the most frequently found. The recognition of microorganisms by innate immune cells is a crucial event for the defense against fungal pathogens. In this scenario, the role of Dectin-1 receptor and its interaction with the β-glucans present in the fungal wall in the host defense against C. albicans, remain unclear. Objective: Our aim was to characterize the Dectin-1/β-glucan interaction in order to evaluate the impact of host and pathogen defective recognition in the outcome of C.albicans systemic infection. Methods: Male C57BL/6 (WT) and Dectin-1 Knock-out (KO)(Clec7a-/-) mice were injected intravenously (iv) with C.albicans SC5314 (parental strain) and identified as WT-SC5314 and KO-SC5314 groups respectively. Beside, Male C57BL/6 (WT) were iv infected with a beta-1,3-D-glucansintase defective strain, C. albicans-FKS1-S645 (WT-FKS645 group). All animals were inoculated with 2.5x106 yeast and at 4h, 12h, 24h, 48h, 7 and 15 days (d) post-infection (pi), the animals were sacrificed. Index of Body Weight (IBW), fungal burden (CFU), histological studies and survival of all animal groups were evaluated.Results: Animals of WT-SC5314 and WT-FKS645 groups showed similar profile of infection with significant decrease of IBW compared with WT-uninfected animals (4h,24h,7d p