CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Histone acetylation and DNA methylation inhibitors active PSG gene expresión.
Autor/es:
CAMOLOTTO SOLEDAD; RACCA ANA; PATRITO LUIS; GENTI-RAIMONDI SUSANA; PANZETTA-DUTARI GRACIELA
Lugar:
San Miguel de Tucumán, Tucumán
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Institución organizadora:
SAIB
Resumen:
The human pregnancy-specific glycoprotein (PSG) gene family is composed by 11 highly similar members whose expression constitutes an early biochemical marker of trophoblast differentiation. They are transcriptionally up-regulated during this process and individual PSG members reach different expression levels. Herein, we study the contribution of epigenetic regulation to PSG expression through histone acetylation and DNA methylation. For this purpose, primary cytotrophoblasts, choriocarcinoma JEG3 and cervix carcinoma HeLa cell lines were treated with histone deacetylase inhibitors, Trichostatin A (TSA) and sodium butyrate (NaBu), and an hypomethylating drug, 5-Aza-Deoxycytidine (aza). Both, PSG mRNA and protein levels were dose-dependently induced, as revealed by immunofluorescence and qRT-PCR assays. TSA and NaBu treatment of JEG3 cells transiently transfected with PSG3 promoter constructs enhanced reporter activity. PSG3 promoter construct bearing a 130 bp promoter sequence overlapping the gene transcription start site. Our results indicate that epigenetic mechanisms are involved in PSG gene regulation, and suggest that the proximal promoter region is recognized by transcription factors which can mediate histone modifications. Supported by CONICET, FONCyT, MinCyT of Córdoba and SECyT UNC.