Association of the Low Density Lipoprotein Receptor-related Protein 1 (LRP1) expression with Diabetic Macular Oedema.

SANCHEZ MC; LORENC VE; COLLINO C; BARONI V; FORZINETTI G; FERRER D; GRAMAJO L; LUNA JD

Fort lauderdale

Otro; ARVO´s 2009 Annual Meeting (The Association for Research in Vision and Ophthalmology).; 2009

Purpose: Diabetic macular edema is a common phenomenon associated with visual loss in diabetic patients and is characterized by accumulation of extracellular fluid in the retina. Up to now, the etiopathogenesis of this disease is not well known which will be essential for the development of an effective clinical treatment. The objective of this work was to determine different biochemical parameters in order to discriminate diabetic patients with clinically significant macular edema (CSMO) from diabetic patients  without CSMO. Methods: Peripheral whole blood samples were obtained of patients with CSMO (n=27), DM patients without retinopathy (n=14) and controls (n=16). Quantitative assays were performed for more than 22 different biochemical parameters measured by conventional clinical laboratory tests. LRP1 together with CD4, CD3 and TLCD4+CD8+ were determined in peripheral blood monocytes by flow cytometry using the flow cytometer Cytoron Absolute (Ortho Diagnostic System, Raritan, NJ), operated with Immunocount II software. The data were analyzed by WinMDI 2.8 software. All biochemical parameters were analyzed using Lineal Discriminant Analysis (forward stepwise mode) and Kruskal Wallis (Statistica version 7.0). Results: Discriminant analysis allows selecting 18 biochemical parameters as the most important for discrimination. These 18 parameters allow differentiating CSMO patients, DM patients and controls with 86,4% certainty. This means that only 8 of 57 patients analyzed were misclassified. The mean value of Red cells, haemoglobin, haematocrit, lymphocytes, HDL cholesterol, triglicerides, glucose and HbA1c was statistically different between groups of patients (p¡Ü0.05). Conclusion: Taken together all biochemical parameters allowed to statistically discrimination between CSMO from the two other patient groups.