Biochemical Parameters Discriminate Diabetic Patients With Clinically Significant Macular Edema (CSME) From Diabetic Patients Without CSME.

SANCHEZ MC; COLLINO C; BARONI V; FORZINETTI G; FERRER D; CHIABRANDO GA; GRAMAJO L; JUAREZ CP; LUNA JD

Fort Lauderdale

Otro; ARVO´s 2010 Annual Meeting (The Association for Research in Vision and Ophthalmology).; 2010

Biochemical Parameters Discriminate Diabetic Patients with Clinically Significant Macular Edema (CSME) from Diabetic Patients without CSME. Author Block M.C. Sanchez1, C.J.G. Collino2, V.M. Baroni3, G. Forzinetti4, D. Ferrer4, G.A. Chiabrando1, L. Gramajo5, C.P. Juarez5, L.G. Garcia5, J.D. Luna5. 1CIBICI-Dpto de Bioquimica Clinica, Fac de Ciencias Quimicas UNC, Cordoba, Argentina; 2CEQUIMAP, FCQ, UNC, Cordoba, Argentina; 3Instituto Superior en Investigaci¨®n, Desarrollo y Servicios en Alimentos, ISIDSA, SECyT, CONICET - UNC, Cordoba, Argentina; 4Laboratorio Hospital Privado, Cordoba, Argentina; 5Dpto de Oftalmolog¨ªa, Fundaci¨®n VER, Cordoba, Argentina.Abstract:Purpose: Diabetic macular edema is a common phenomenon associated with visual loss in diabetic patients and is characterized by accumulation of extracellular fluid in the retina. Up to now, the etiopathogenesis of this disease is not well known, which makes difficult the development of an effective treatment. The objective of this work was to determine different biochemical parameters in order to discriminate diabetic patients with clinically significant macular edema (CSME) from diabetic patients without CSME.Methods: Peripheral whole blood samples were obtained of diabetic patients with CSME (n=27), diabetic patients without CSME and retinopathy (n=14) and controls (n=16). Quantitative assays were performed for 18 different biochemical parameters measured by conventional clinical laboratory tests with the exception of monocyte LRP1 and T lymphocyte (TL) surface antigens, including TLCD3+, TLCD4+, and TLCD4+CD8+, which were determined by flow cytometry using a Cytoron Absolute (Ortho Diagnostic System, Raritan, NJ), operated with Immunocount II software. The data were analyzed by WinMDI 2.8 software. All biochemical parameters were analyzed using Lineal Discriminant Analysis and Kruskal Wallis (Statistica version 7.0).Results: Discriminant analysis applied on these 18 parameters allow differentiating diabetic patients with CSME, diabetic patients without CSME and controls with 86.4% certainty. This means that only 8 of 57 patients analyzed were misclassified. The mean value of Red cells, haemoglobin, haematocrit, lymphocytes, HDL cholesterol, triglicerides, glucose and HbA1c was statistically different between groups of patients (p¡Ü0.05).Conclusions: Taken together all biochemical parameters allowed to statistically discrimination between CSME from the two other patient groups.:Author Disclosure Information: M.C. Sanchez, None; C.J.G. Collino, None; V.M. Baroni, None; G. Forzinetti, None; D. Ferrer, None; G.A. Chiabrando, None; L. Gramajo, None; C.P. Juarez, None; L.G. Garcia, None; J.D. Luna, None.