DIFFERENTIAL LOCALIZATION OF CD73 IN TARGET TISSUES OF TRYPANOSOMA CRUZI INFECTION AND THE EFFECT OF THIS ADENOSINE GENERATING ECTOENZYME ON LOCAL PARASITE REPLICATION

MAZZOCO YANINA; BERGERO GASTÓN; THEUMER MARTÍN GUSTAVO; EBERHARDT NATALIA; AOKI PILAR

Tucumán

Congreso; LXVII Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2019

Sociedad Argentina de Inmunología

Growing evidence demonstrates a critical role of purinergic system in the modulation of theimmune response and tissue repair. Damaged cells release the pro-inflammatory moleculeATP, which is metabolized by the ectonucleotidases CD39 and CD73 to the antiinflammatory mediator adenosine (ADO). Thus, the balance between ATP/ADO is knownto determine the outcome of inflammation/infection. Here, we explored the cellular sourceof CD73 ectoenzyme expression in different Trypanosoma cruzi infected tissues and theimpact of its activity in local parasite replication. Through confocal analysis ofimmunofluorescence assays we found that immune cells exhibited significantly higherlevels of CD73 expression than parenchymal cells at 7 days post-infection (dpi), whilecardiomyocytes, adipocytes and hepatocytes expressed lower amount of this ectoenzyme(p