Study of the role of tissue resident regulatory T cells during Trypanosoma cruzi infection.

RODRIGUEZ C; GRUPPI A; BOCCARDO S; ARAUJO FURLÁN CL; ABRATE C; ALMADA L; MONTES CL; ACOSTA RODRIGUEZ EV

Congreso; SAIC-SAI-SAFIS 2018.; 2018

During T. cruzi (Tc) infection, a limited CD4+Foxp3+ regulatoryT cells (Tregs) response allows the induction ofprotective CD8+ T cell immunity in peripheral tissues, butit may also facilitate tissue damage and immunopathology.Tissue resident Tregs (tisTregs) are a specializedsubset that, besides regulating effector cells, maintainstissue homeostasis. We previously showed that Tc infectedmice exhibit a reduced frequency of Tregs, particularlyof those expressing the tisTregs markers ST2 and KLRG-1, in Blood, Spleen, Liver and Skeletal Muscle (SM).Our current aim is to evaluate concentration of tisTregsgrowth factors during Tc infection and the role of TisTregsin the regulation of effector immune responses and immunopathology.To this end, Foxp3-GFP-C57BL/6 miceinfected with 5000 Tc parasites (Tulahuen) were usedas infection model. The levels of IL-33 and IL-18, recognizedtisTregs growth factors, were determined by ELISAin plasma, and Spleen and SM lysates. IL-33 levels decreasedand IL-18 levels increased after 14 and 21 dpiin plasma and spleen, but both cytokines increased after21dpi in SM (p