Study of the IL-17 family cytokine and receptor expression on different tumor cell lines and their impact on tumor progression.

RODRIGUEZ C; CANALE FP; BECCARIA CG; ACOSTA RODRIGUEZ EV; ARAUJO FURLÁN CL; TOSELLO BOARI J; BOSSIO SN; BOCCARDO S; MONTES CL; GRUPPI A

Congreso; SAIC-SAI-SAFIS 2018.; 2018

While relatively well-defined in infectious diseases and autoimmunity,the roles of IL-17 cytokines in cancer remain controversial. Thus,IL-17 may play a pro-tumoral role by directly sustaining cancer cellgrowth or may support antitumoral immunity potentiating CD8+ Tcell (CTL) and NK cell responses. Our aim is to determine the roleof IL-17 in the overall progression of different cancer types, dissectingpro-tumoral and anti-tumoral effects. Using different murinetumor cell lines of melanoma (B16-SIY), fibrosarcoma (MC57-SIYand MCA-OVA), lymphoma (EL4-SIY) and acute myeloid leukemia(C1498-SIY), we first quantified by real-time PCR the transcripts encodingIL-17A and IL-17F and receptors subunits IL-17RA, IL-17RCand IL-17RD. IL-17A transcript was detected only in EL4-SIY whileIL-17RA, IL-17RD and IL-17F mRNA were expressed in all the celllines assayed. IL-17RC transcripts were expressed in MCA-OVA,B16-SIY and MC57-SIY but not in EL4-SIY nor in C1498-SIY. Importantvariations in transcript amounts were observed among celllines evaluated: IL-17F mRNA expression was higher in C1498-SIY(p< 0.001) and B16-SIY (p