CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Candida albicans activates type I interferon pathway in cells of the female genital tract
Autor/es:
MIRÓ, MARÍA SOLEDAD; VIGEZZI, CECILIA; SOTOMAYOR, CLAUDIA ELENA; RODRIGUEZ, EMILSE; ICELY, PAULA ALEJANDRA
Reunión:
Congreso; LXVI Annual Meeting of Sociedad Argentina de Inmunología; 2018
Resumen:
C. albicans (Ca) is an opportunistic fungal pathogen that typically exists as a harmless commensal in the female genital tract; however, approximately 75% of women who are of reproductive-age suffer from vulvovaginal candidiasis(VVC) at least once in their lifetime. Recent studies revealed that, besides viruses and bacteria, fungal pathogens can also induce type I interferons (IFNs-I) production by innate immune cells. The aim was to study whether Ca recognition induces IFNs-I in epithelial cells of female genital tract in order to establish a possible role during VVC. For this purpose, human cervical epithelial cell line (HeLa) were stimulated with: Ca SC5314 strain as Invasive Ca (hypha emission) and Non-invasive Ca (pseudomicelio) (0,25:1 fungus:cell ratio); Ca DNA complexed with polyethylenimine (Ca-DNA), LPS and Poly I:C during 4 and 24h. IFN, IRF3, IRF7 and MX1 (IFNAR receptor activation marker) mRNA levels were measured by qPCR. A comparative analysis was made between 4 and 24h. LPS (4h) and Poly I:C (24h) were able to induce a strong IFN mRNA expression in HeLa cells(p