In vivo role of IFN-y-mediated skin immune response in experimental dermatophytosis in IL-17RA deficient mice

GUASCONI L; GRUPPI A; BECCACECE I; ORELLANO A; CHIAPELLO LAURA S.; BURSTEIN VL; MENA C; THEUMER MG

Mar del Plata

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología. SAI-SAIC-SAFIS; 2018

Sociedad Argentina de Inmunología. SAI-SAIC-SAFIS

Microsporum canis is a dermatophyte fungus that causes superficial infections and is highly prevalent among immunocompetent children. Previously, we demonstrated that M. canis induces a type 17 immunity in vivo that controls fungal proliferation in skin and down-modulates an antigen-specific IFN-γ response.Objective: To evaluate the role of IFN-γ in the experimental dermatophytosis outcome in absence of IL-17RA signaling.Wild type (WT) and IL-17RA-deficient C57BL/6 (KO) mice were epicutaneously infected with M. canis hyphae. On different days post-infection (dpi), histopathology, CD11b+Ly6G+ population (neutrophils, FACS), cytokine analysis (ELISA, FACS) and fungal burden (colony forming units/ g skin) were determined in epidermis. For IFN-γ blocking, anti IFN-γ antibody (BioXCell, 400 µg/mice) or isotype control (IC) was injected (intraperitoneally) on 3 and 6 dpi. We observed that by 6 and 8 dpi, infected KO showed an increase in fungal burden (p