CHARACTERIZATION OF RETINALS FUNCIONALS ASPECT IN A MOUSE MODEL OF LASER-INDUCED CHOROIDAL NEOVASCULARIZATION (CNV)

SUBIRADA PV; VAGLIENTI MV; PAZ MC; BARCELONA PF; SADDI TN; MARQUEZ AM; RIDANO, ME; MARQUEZ GE; LUNA JD; SANCHEZ MC

Cordoba

Congreso; SAN 2018 XXXIII Congreso Anual de Sociedad Argentina de Investigación en Neurociencias; 2018

Age-related macular degeneration (AMD) in its Choroidal neovascularization (CNV) form is the leading cause of vision loss among adults. In the present study, we validate an established CNV mice model. In order to characterize the neovascular process, emphasizing the participation of the α2M/LRP1 system as well as inflammatory profile and retinal functionality. Previously, we demonstrated that α2M and its receptor, LRP1 participate during retinal NV and neurodegenerative process in different retinopathies. C57BL/6 mice, were treated with four spots of argon green laser photocoagulation per eye. After 7 days of laser, we analyzed the NV on choroid?RPE flatmounts by isolectin B4 staining and cell markers we characterize cells in the lesion: CD105 (ECs), NG2 (pericytes), F4/80 and Iba1 (microglia), by IF, and LRP1 cell distribution by confocal microscopy. In addition, the protein levels of α2M and LRP1 were analyzed by WB, the inflammatory and pro- angiogenic profile by qPCR assay, and retinal functionality by scotopic ERG. We had able to standardize the size of the lesion. An important number of ECs, pericyte and microglia was observed around the lesion. While α2M and LRP1 showed high expression pattern on cells close to the lesion. Accompanied with high pro-inflammatory and pro-angiogenic factors. The scotopic ERG a- and b-wave were decreased in this animals vs control. In conclusion, we were able to reproduce the CNV model, to evaluate the participation of α2M/LRP1 system.