CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HIF-1a/ purinergic axis is altered in patients with chronic Chagas disease
Autor/es:
SANMARCO, LILIANA MARIA; ANGEL RAMÓN MINGUEZ; EBERHARDT, NATALIA; GASTÓN BERGERO; LAURA MARINA VISCONTI; CARLOS ADOLFO VIGLIANO; AOKI, MARIA PILAR
Lugar:
Mar del Plata
Reunión:
Congreso; SAIC SAI SAFIS 2018; 2018
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Introduction: The molecular mechanisms involved in the development of human chronic Chagas cardiomyopathy (CCC) are still largely unknown. Purinergic system components have taken a robust significance as danger signals and modulators of immunity. Ischemic cells release ATP (inflammasome activator), which is metabolized by CD39/CD73 ectoenzymes to anti-inflammatory adenosine. We have reported that CD73 pharmacological inhibition during acute T. cruzi murine infection reduced progression of CCC. The aim of this study was to explore the hypoxia-inducible factor-1α (HIF-1α)/purinergic system axis in immune cells from infected individuals and in cardiac explants from CCC patients. Materials and Methods: Seropositive patients (n = 24) were evaluated clinically and by electrocardiogram and chest X-ray. The uninfected control group (n = 24) consisted of age-matched individuals. Cardiac tissue samples from end-stage CCC patients were stained by IHC/IF. The myocarditis degree was determined considering the number of CD68+ plus CD3+ cells, as follow: Severe≥ median number; Moderate 25-50th percentile; or Mild≤ 25th percentile. Results: In comparison with control donors, infected patients showed higher frequency of HIF-1α+ and IL-1β+ circulating monocytes with increased nitric oxide production (p