Inhibitory capacity of a Fatty Acid Binding Protein of Fasciola hepatica (Fh12) on the activation of murine mature dendritic cells

ANA M. ESPINO; RUIZ-JIMÉNEZ CALEB; CERVI LAURA; CELIAS DAIANA

Congreso; American Association of Immunology Congress 2017; 2017

Fasciola hepatica, is a helminth that excretes-secretes different proteinsthat modulate cells of the immune system, generating antiinflammatoryresponses. It has been demonstrated that F. hepatica?sfatty acid binding protein (Fh12) achieve this anti-inflammatory effectby inhibiting the expression of TLR4 induced by for LPS inmacrophages by a number of mechanisms that operatesimultaneously. Fh12 target the CD14 co-receptor, thus blocking theLPS-CD14 binding, which stop the entire TLR4 activation cascadefrom the beginning of the LPS-stimuli. Concurrently, Fh12 suppressthe phosphorylation of various kinases (p38, JNK and ERK)downstream the TLR4 signaling cascade and induce the activation ofmacrophages by an alternative pathway. Dendritic cells (DCs) areantigen-presenting cells that play key role at early phase of innateimmunity and that are essential in the development of adaptiveimmune response. The main objective of the current study was toinvestigate the effect of Fh12 on the activation of murine DCs maturedin the presence of LPS. DCs were isolated from bone marrow of naïveC57BL / 6 mice, cultured, differentiated and stimulated in vitro withFh12 1h prior to stimulation with LPS for 18 hrs. ELISA was used toquantify the amount of a panel of secreted cytokines in culturesupernatant. FACS was used to determine the activation of MHCIIand co-stimulatory molecules. Results demonstrated that Fh12significantly inhibit the production of IL-12p70 (p < 0.03) and IL-6(p